Quantifying vemurafenib in dried blood spots using high-performance LC-MS/MS

Cynthia M Nijenhuis; Hilde Rosing; Jan H M Schellens; Jos H Beijnen

doi:10.4155/bio.14.171

Quantifying vemurafenib in dried blood spots using high-performance LC-MS/MS

Cynthia M Nijenhuis, Hilde Rosing, Jan H M Schellens, Jos H Beijnen

Pharmacoepidemiology and Clinical Pharmacology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: To further investigate the pharmacokinetics of vemurafenib and to support therapeutic drug monitoring an LC-MS/MS method was developed and validated for the quantification of vemurafenib in dried blood spots.

RESULTS: Vemurafenib was extracted from the dried blood spots by methanol:acetonitrile (50:50, v/v) and separated on a C18 column with gradient elution and analyzed with triple quadrupole mass spectrometry in positive ion mode. The validated calibration range is linear from 1 to 100 µg/ml. Intra- and inter-assay accuracies and precisions were within ±13.6% and ≤6.5%. The applicability of the assay was tested by analyzing dried blood spots samples of melanoma patients receiving vemurafenib.

CONCLUSION: This assay met all predefined validation criteria and is considered suitable to quantify vemurafenib in dried blood samples.

Original language	English
Pages (from-to)	3215-3224
Number of pages	10
Journal	Bioanalysis
Volume	6
Issue number	23
DOIs	https://doi.org/10.4155/bio.14.171
Publication status	Published - Dec 2014

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title = "Quantifying vemurafenib in dried blood spots using high-performance LC-MS/MS",

abstract = "BACKGROUND: To further investigate the pharmacokinetics of vemurafenib and to support therapeutic drug monitoring an LC-MS/MS method was developed and validated for the quantification of vemurafenib in dried blood spots.RESULTS: Vemurafenib was extracted from the dried blood spots by methanol:acetonitrile (50:50, v/v) and separated on a C18 column with gradient elution and analyzed with triple quadrupole mass spectrometry in positive ion mode. The validated calibration range is linear from 1 to 100 µg/ml. Intra- and inter-assay accuracies and precisions were within ±13.6% and ≤6.5%. The applicability of the assay was tested by analyzing dried blood spots samples of melanoma patients receiving vemurafenib.CONCLUSION: This assay met all predefined validation criteria and is considered suitable to quantify vemurafenib in dried blood samples.",

author = "Nijenhuis, {Cynthia M} and Hilde Rosing and Schellens, {Jan H M} and Beijnen, {Jos H}",

year = "2014",

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T1 - Quantifying vemurafenib in dried blood spots using high-performance LC-MS/MS

AU - Nijenhuis, Cynthia M

AU - Rosing, Hilde

AU - Schellens, Jan H M

AU - Beijnen, Jos H

PY - 2014/12

Y1 - 2014/12

N2 - BACKGROUND: To further investigate the pharmacokinetics of vemurafenib and to support therapeutic drug monitoring an LC-MS/MS method was developed and validated for the quantification of vemurafenib in dried blood spots.RESULTS: Vemurafenib was extracted from the dried blood spots by methanol:acetonitrile (50:50, v/v) and separated on a C18 column with gradient elution and analyzed with triple quadrupole mass spectrometry in positive ion mode. The validated calibration range is linear from 1 to 100 µg/ml. Intra- and inter-assay accuracies and precisions were within ±13.6% and ≤6.5%. The applicability of the assay was tested by analyzing dried blood spots samples of melanoma patients receiving vemurafenib.CONCLUSION: This assay met all predefined validation criteria and is considered suitable to quantify vemurafenib in dried blood samples.

AB - BACKGROUND: To further investigate the pharmacokinetics of vemurafenib and to support therapeutic drug monitoring an LC-MS/MS method was developed and validated for the quantification of vemurafenib in dried blood spots.RESULTS: Vemurafenib was extracted from the dried blood spots by methanol:acetonitrile (50:50, v/v) and separated on a C18 column with gradient elution and analyzed with triple quadrupole mass spectrometry in positive ion mode. The validated calibration range is linear from 1 to 100 µg/ml. Intra- and inter-assay accuracies and precisions were within ±13.6% and ≤6.5%. The applicability of the assay was tested by analyzing dried blood spots samples of melanoma patients receiving vemurafenib.CONCLUSION: This assay met all predefined validation criteria and is considered suitable to quantify vemurafenib in dried blood samples.

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DO - 10.4155/bio.14.171

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VL - 6

SP - 3215

EP - 3224

JO - Bioanalysis

JF - Bioanalysis

IS - 23

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Quantifying vemurafenib in dried blood spots using high-performance LC-MS/MS

Abstract

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