Quantification of Receptor Association, Dissociation, and NA-Dependent Motility of Influenza A Particles by Biolayer Interferometry

Erik de Vries; Hongbo Guo; Wenjuan Du; Mengying Liu; Frank J M van Kuppeveld; Cornelis A M de Haan

doi:10.1007/978-1-0716-2635-1_10

Quantification of Receptor Association, Dissociation, and NA-Dependent Motility of Influenza A Particles by Biolayer Interferometry

Erik de Vries
, Hongbo Guo
, Wenjuan Du
, Mengying Liu
, Frank J M van Kuppeveld
, Cornelis A M de Haan

Virology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

We describe a method for real-time analysis and quantification of influenza A virus (IAV)-receptor interactions by biolayer interferometry (BLI). Biotinylated synthetic sialoglycans or sialoglycoproteins (biotinylated or Fc-tagged) were immobilized on the tip of biosensors (coated with streptavidin or protein A) that were subsequently dipped into IAV particle solutions in 96-well plates. Association and/or dissociation of IAV particles was recorded in consecutive steps in buffers of choice. From the association and dissociation curves, parameters can be derived that describe IAV particle-receptor interactions in absence or presence of neuraminidase activity. Overall, the method provides a quantitative description of the hemagglutinin-neuraminidase balance that determines the interaction kinetics of IAV with specific sialoglycan receptors.

Original language	English
Title of host publication	Glycovirology
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press
Chapter	10
Pages	123-140
Number of pages	18
ISBN (Electronic)	978-1-0716-2635-1
ISBN (Print)	978-1-0716-2634-4
DOIs	https://doi.org/10.1007/978-1-0716-2635-1_10
Publication status	Published - 30 Sept 2022

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	2556
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Neuraminidase
Binding kinetics
Hemagglutinin
Biolayer interferometry
Virus motility
Association
Influenza virus
Sialoglycoprotein
Sialoglycan
Dissociation

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10.1007/978-1-0716-2635-1_10

978-1-0716-2635-1_10Final published version, 594 KBLicence: Taverne

Cite this

de Vries, E., Guo, H., Du, W., Liu, M., van Kuppeveld, F. J. M., & de Haan, C. A. M. (2022). Quantification of Receptor Association, Dissociation, and NA-Dependent Motility of Influenza A Particles by Biolayer Interferometry. In Glycovirology: Methods and Protocols (pp. 123-140). (Methods in Molecular Biology; Vol. 2556). Humana Press. https://doi.org/10.1007/978-1-0716-2635-1_10

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abstract = "We describe a method for real-time analysis and quantification of influenza A virus (IAV)-receptor interactions by biolayer interferometry (BLI). Biotinylated synthetic sialoglycans or sialoglycoproteins (biotinylated or Fc-tagged) were immobilized on the tip of biosensors (coated with streptavidin or protein A) that were subsequently dipped into IAV particle solutions in 96-well plates. Association and/or dissociation of IAV particles was recorded in consecutive steps in buffers of choice. From the association and dissociation curves, parameters can be derived that describe IAV particle-receptor interactions in absence or presence of neuraminidase activity. Overall, the method provides a quantitative description of the hemagglutinin-neuraminidase balance that determines the interaction kinetics of IAV with specific sialoglycan receptors.",

keywords = "Neuraminidase, Binding kinetics, Hemagglutinin, Biolayer interferometry, Virus motility, Association, Influenza virus, Sialoglycoprotein, Sialoglycan, Dissociation",

author = "\{de Vries\}, Erik and Hongbo Guo and Wenjuan Du and Mengying Liu and \{van Kuppeveld\}, \{Frank J M\} and \{de Haan\}, \{Cornelis A M\}",

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Quantification of Receptor Association, Dissociation, and NA-Dependent Motility of Influenza A Particles by Biolayer Interferometry. / de Vries, Erik; Guo, Hongbo; Du, Wenjuan et al.
Glycovirology: Methods and Protocols. Humana Press, 2022. p. 123-140 (Methods in Molecular Biology; Vol. 2556).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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AU - Liu, Mengying

AU - van Kuppeveld, Frank J M

AU - de Haan, Cornelis A M

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AB - We describe a method for real-time analysis and quantification of influenza A virus (IAV)-receptor interactions by biolayer interferometry (BLI). Biotinylated synthetic sialoglycans or sialoglycoproteins (biotinylated or Fc-tagged) were immobilized on the tip of biosensors (coated with streptavidin or protein A) that were subsequently dipped into IAV particle solutions in 96-well plates. Association and/or dissociation of IAV particles was recorded in consecutive steps in buffers of choice. From the association and dissociation curves, parameters can be derived that describe IAV particle-receptor interactions in absence or presence of neuraminidase activity. Overall, the method provides a quantitative description of the hemagglutinin-neuraminidase balance that determines the interaction kinetics of IAV with specific sialoglycan receptors.

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KW - Binding kinetics

KW - Hemagglutinin

KW - Biolayer interferometry

KW - Virus motility

KW - Association

KW - Influenza virus

KW - Sialoglycoprotein

KW - Sialoglycan

KW - Dissociation

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BT - Glycovirology

PB - Humana Press

ER -

Quantification of Receptor Association, Dissociation, and NA-Dependent Motility of Influenza A Particles by Biolayer Interferometry

Abstract

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Keywords

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