Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloids

Giulia Perticari; Maroussia M.P. Ganpat; Jarno Drost

doi:10.1016/j.xpro.2026.104362

Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloids

Giulia Perticari
, Maroussia M.P. Ganpat
, Jarno Drost^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Organoids derived from normal human tissue have proven to be effective preclinical models for studying tumor progression through the introduction of driver mutations. In this protocol, we outline techniques for disease modeling using organoids derived from healthy kidney tissue, also known as tubuloids. We provide step-by-step instructions for the establishment and passaging of tubuloids, lentiviral transduction, processing for histological analysis, and preparation for CUT&RUN and single-cell transcriptomic profiling. For complete details on the use and execution of this protocol, please refer to Ganpat et al.

Original language	English
Article number	104362
Number of pages	21
Journal	STAR Protocols
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2026.104362
Publication status	Published - 20 Mar 2026

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Publisher Copyright:
© 2026 The Author(s).

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloidsFinal published version, 12.4 MBLicence: CC BY

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title = "Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloids",

abstract = "Organoids derived from normal human tissue have proven to be effective preclinical models for studying tumor progression through the introduction of driver mutations. In this protocol, we outline techniques for disease modeling using organoids derived from healthy kidney tissue, also known as tubuloids. We provide step-by-step instructions for the establishment and passaging of tubuloids, lentiviral transduction, processing for histological analysis, and preparation for CUT\&RUN and single-cell transcriptomic profiling. For complete details on the use and execution of this protocol, please refer to Ganpat et al.",

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AB - Organoids derived from normal human tissue have proven to be effective preclinical models for studying tumor progression through the introduction of driver mutations. In this protocol, we outline techniques for disease modeling using organoids derived from healthy kidney tissue, also known as tubuloids. We provide step-by-step instructions for the establishment and passaging of tubuloids, lentiviral transduction, processing for histological analysis, and preparation for CUT&RUN and single-cell transcriptomic profiling. For complete details on the use and execution of this protocol, please refer to Ganpat et al.

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Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloids

Abstract

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