TY - JOUR
T1 - Proteomic Profiling of Mouse Helper T Cell Differentiation
AU - van den Ham, Henk-Jan
AU - Binai, Nadine A
AU - Zaaraoui-Boutahar, Fatiha
AU - Heck, Albert J R
AU - Andeweg, Arno C
N1 - This article is protected by copyright. All rights reserved.
PY - 2019/4
Y1 - 2019/4
N2 - Helper T cell differentiation is a key process in the regulation of adaptive immune responses. Here, we profile mouse Th1 and Th2 cells using high-throughput proteomics to increase our understanding of the molecular biology of Th differentiation to support the design of prophylactic and therapeutic intervention strategies for (infectious) diseases. Protein profiling of Th1/Th2 differentiated cells resulted in the quantification of almost 6000 proteins of which 41 were differentially expressed at FDR < 0.1, and 19 at the FDR < 0.05 level, respectively. Differential protein expression analysis identifies a number of the expected canonical Th differentiation markers, and gene set analysis using the REACTOME database and a hypergeometric test (FDR < 0.05) confirms that helper T cell pathways are the top sets that are differentially expressed. Additionally, by network analysis we are able to associate many differentially expressed proteins to the Th1 and Th2 pathways. This article is protected by copyright. All rights reserved.
AB - Helper T cell differentiation is a key process in the regulation of adaptive immune responses. Here, we profile mouse Th1 and Th2 cells using high-throughput proteomics to increase our understanding of the molecular biology of Th differentiation to support the design of prophylactic and therapeutic intervention strategies for (infectious) diseases. Protein profiling of Th1/Th2 differentiated cells resulted in the quantification of almost 6000 proteins of which 41 were differentially expressed at FDR < 0.1, and 19 at the FDR < 0.05 level, respectively. Differential protein expression analysis identifies a number of the expected canonical Th differentiation markers, and gene set analysis using the REACTOME database and a hypergeometric test (FDR < 0.05) confirms that helper T cell pathways are the top sets that are differentially expressed. Additionally, by network analysis we are able to associate many differentially expressed proteins to the Th1 and Th2 pathways. This article is protected by copyright. All rights reserved.
U2 - 10.1002/pmic.201800045
DO - 10.1002/pmic.201800045
M3 - Article
C2 - 30758134
SN - 1615-9853
VL - 19
SP - e1800045
JO - Proteomics
JF - Proteomics
IS - 7
ER -
