Protein versus DNA as a marker for peripheral blood mononuclear cell counting

R.S. Jansen; H. Rosing; J.H.M. Schellens; J.H. Beijnen

Protein versus DNA as a marker for peripheral blood mononuclear cell counting

R.S. Jansen, H. Rosing, J.H.M. Schellens, J.H. Beijnen

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Abstract

Quantitative analysis of intracellular analytes requires an accurate and precise assay not only for the quantitation of the analytes, but also for the quantitation of the number of cells in which they were determined. In this technical note we compare protein and DNA as markers for the number of peripheral blood mononuclear cells (PBMCs) isolated from whole blood. The protein content of samples was highly influenced by red blood cell contamination and was, therefore, a less suitable marker. The DNA-based method was unaffected by red blood cell contamination and was finally validated over a range from 10 x 10(6) to 300 x 10(6) PBMCs/mL.

Original language	Undefined/Unknown
Pages (from-to)	863-867
Number of pages	5
Journal	Analytical and Bioanalytical Chemistry
Volume	395
Issue number	3
Publication status	Published - 2009

Cite this

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title = "Protein versus DNA as a marker for peripheral blood mononuclear cell counting",

abstract = "Quantitative analysis of intracellular analytes requires an accurate and precise assay not only for the quantitation of the analytes, but also for the quantitation of the number of cells in which they were determined. In this technical note we compare protein and DNA as markers for the number of peripheral blood mononuclear cells (PBMCs) isolated from whole blood. The protein content of samples was highly influenced by red blood cell contamination and was, therefore, a less suitable marker. The DNA-based method was unaffected by red blood cell contamination and was finally validated over a range from 10 x 10(6) to 300 x 10(6) PBMCs/mL.",

author = "R.S. Jansen and H. Rosing and J.H.M. Schellens and J.H. Beijnen",

note = "ISI:000269916200030 TiPharma Spring meeting",

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T1 - Protein versus DNA as a marker for peripheral blood mononuclear cell counting

AU - Jansen, R.S.

AU - Rosing, H.

AU - Schellens, J.H.M.

AU - Beijnen, J.H.

N1 - ISI:000269916200030 TiPharma Spring meeting

PY - 2009

Y1 - 2009

N2 - Quantitative analysis of intracellular analytes requires an accurate and precise assay not only for the quantitation of the analytes, but also for the quantitation of the number of cells in which they were determined. In this technical note we compare protein and DNA as markers for the number of peripheral blood mononuclear cells (PBMCs) isolated from whole blood. The protein content of samples was highly influenced by red blood cell contamination and was, therefore, a less suitable marker. The DNA-based method was unaffected by red blood cell contamination and was finally validated over a range from 10 x 10(6) to 300 x 10(6) PBMCs/mL.

AB - Quantitative analysis of intracellular analytes requires an accurate and precise assay not only for the quantitation of the analytes, but also for the quantitation of the number of cells in which they were determined. In this technical note we compare protein and DNA as markers for the number of peripheral blood mononuclear cells (PBMCs) isolated from whole blood. The protein content of samples was highly influenced by red blood cell contamination and was, therefore, a less suitable marker. The DNA-based method was unaffected by red blood cell contamination and was finally validated over a range from 10 x 10(6) to 300 x 10(6) PBMCs/mL.

M3 - Article

SN - 1618-2642

VL - 395

SP - 863

EP - 867

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 3

ER -