Abstract
Photoactivatable groups meeting the criterion of minimal
perturbance allow the investigation of interactions in
biological samples. Here, we review the application of
photoactivatable groups in lipids enabling the study of
protein-lipid interactions in (biological) membranes. The
chemistry of various photoactivatable groups is summarized
and the specificity of the interactions detected
is discussed. The recent introduction of ‘click chemistry’
in photocrosslinking of membrane proteins by photoactivatable
lipids opens new possibilities for the analysis
of crosslinked products and will help to close the gap
between proteomics and lipidomics.
| Original language | English |
|---|---|
| Pages (from-to) | 795-803 |
| Number of pages | 9 |
| Journal | Biological Chemistry |
| Volume | 390 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2009 |