Protein histochemistry using coronaviral spike proteins: studying binding profiles and sialic acid requirements for attachment to tissues

Iresha Ambepitiya Wickramasinghe; M Hélène Verheije

doi:10.1007/978-1-4939-2438-7_14

Protein histochemistry using coronaviral spike proteins: studying binding profiles and sialic acid requirements for attachment to tissues

Iresha Ambepitiya Wickramasinghe, M Hélène Verheije

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Protein histochemistry is a tissue-based technique that enables the analysis of viral attachment patterns as well as the identification of specific viral and host determinants involved in the first step in the infection of a host cell by a virus. Applying recombinantly expressed spike proteins of infectious bronchitis virus onto formalin-fixed tissues allows us to profile the binding characteristics of these viral attachment proteins to tissues of various avian species. In particular, sialic acid-mediated tissue binding of spike proteins can be analyzed by pretreating tissues with various neuraminidases or by blocking the binding of the viral proteins with specific lectins. Our assay is particularly convenient to elucidate critical virus-host interactions for viruses for which infection models are limited.

Original language	English
Pages (from-to)	155-163
Number of pages	9
Journal	Methods in Molecular Biology
Volume	1282
DOIs	https://doi.org/10.1007/978-1-4939-2438-7_14
Publication status	Published - 2015

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title = "Protein histochemistry using coronaviral spike proteins: studying binding profiles and sialic acid requirements for attachment to tissues",

abstract = "Protein histochemistry is a tissue-based technique that enables the analysis of viral attachment patterns as well as the identification of specific viral and host determinants involved in the first step in the infection of a host cell by a virus. Applying recombinantly expressed spike proteins of infectious bronchitis virus onto formalin-fixed tissues allows us to profile the binding characteristics of these viral attachment proteins to tissues of various avian species. In particular, sialic acid-mediated tissue binding of spike proteins can be analyzed by pretreating tissues with various neuraminidases or by blocking the binding of the viral proteins with specific lectins. Our assay is particularly convenient to elucidate critical virus-host interactions for viruses for which infection models are limited.",

author = "{Ambepitiya Wickramasinghe}, Iresha and Verheije, {M H{\'e}l{\`e}ne}",

year = "2015",

doi = "10.1007/978-1-4939-2438-7_14",

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AU - Verheije, M Hélène

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AB - Protein histochemistry is a tissue-based technique that enables the analysis of viral attachment patterns as well as the identification of specific viral and host determinants involved in the first step in the infection of a host cell by a virus. Applying recombinantly expressed spike proteins of infectious bronchitis virus onto formalin-fixed tissues allows us to profile the binding characteristics of these viral attachment proteins to tissues of various avian species. In particular, sialic acid-mediated tissue binding of spike proteins can be analyzed by pretreating tissues with various neuraminidases or by blocking the binding of the viral proteins with specific lectins. Our assay is particularly convenient to elucidate critical virus-host interactions for viruses for which infection models are limited.

U2 - 10.1007/978-1-4939-2438-7_14

DO - 10.1007/978-1-4939-2438-7_14

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EP - 163

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Protein histochemistry using coronaviral spike proteins: studying binding profiles and sialic acid requirements for attachment to tissues

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