Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR

E. Pacholewicz; A. Swart; L.J.A. Lipman; J.A. Wagenaar; A.H. Havelaar; B. Duim

doi:10.1016/j.mimet.2013.06.003

Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR

E. Pacholewicz, A. Swart, L.J.A. Lipman, J.A. Wagenaar, A.H. Havelaar, B. Duim

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Abstract

A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.

Original language	English
Pages (from-to)	32-38
Number of pages	7
Journal	Journal of Microbiological Methods
Volume	95
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2013.06.003
Publication status	Published - 2013

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title = "Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR",

abstract = "A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.",

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AU - Wagenaar, J.A.

AU - Havelaar, A.H.

AU - Duim, B.

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AB - A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells.

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DO - 10.1016/j.mimet.2013.06.003

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Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR

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