Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry

M G Gerritsen; P Brinkman; Natalia Escobar Salazar; L D Bos; K de Heer; M Meijer; H-G Janssen; H de Cock; H A B Wösten; C.E. Visser; M H J van Oers; P J Sterk

doi:10.1093/mmy/myx035

Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry

M G Gerritsen, P Brinkman, Natalia Escobar Salazar, L D Bos, K de Heer, M Meijer, H-G Janssen, H de Cock, H A B Wösten, C.E. Visser, M H J van Oers, P J Sterk

Sub Molecular Microbiology

University of Amsterdam
Academic Medical Center
Applied and Industrial Mycology, Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands; Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Wytemaweg 80, 3015 CE Rotterdam, The Netherlands.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography-mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo.

Original language	English
Pages (from-to)	253–256
Number of pages	4
Journal	Medical Mycology
Volume	56
Issue number	2
DOIs	https://doi.org/10.1093/mmy/myx035
Publication status	Published - Feb 2018

Keywords

gas chromatography-mass spectrometry
Aspergillus
headspace analysis
exhaled breath analysis
invasive pulmonary aspergillosis

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Gerritsen, M. G., Brinkman, P., Escobar Salazar, N., Bos, L. D., de Heer, K., Meijer, M., Janssen, H.-G., de Cock, H., Wösten, H. A. B., Visser, C. E., van Oers, M. H. J., & Sterk, P. J. (2018). Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry. Medical Mycology, 56(2), 253–256. https://doi.org/10.1093/mmy/myx035

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title = "Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry",

abstract = "Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography-mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo.",

keywords = "gas chromatography-mass spectrometry, Aspergillus, headspace analysis, exhaled breath analysis, invasive pulmonary aspergillosis",

author = "Gerritsen, {M G} and P Brinkman and {Escobar Salazar}, Natalia and Bos, {L D} and {de Heer}, K and M Meijer and H-G Janssen and {de Cock}, H and W{\"o}sten, {H A B} and C.E. Visser and {van Oers}, {M H J} and Sterk, {P J}",

note = "{\textcopyright} The Author 2017. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: [email protected].",

year = "2018",

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doi = "10.1093/mmy/myx035",

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volume = "56",

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Gerritsen, MG, Brinkman, P, Escobar Salazar, N, Bos, LD, de Heer, K, Meijer, M, Janssen, H-G, de Cock, H , Wösten, HAB, Visser, CE, van Oers, MHJ & Sterk, PJ 2018, 'Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry', Medical Mycology, vol. 56, no. 2, pp. 253–256. https://doi.org/10.1093/mmy/myx035

TY - JOUR

T1 - Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry

AU - Gerritsen, M G

AU - Brinkman, P

AU - Escobar Salazar, Natalia

AU - Bos, L D

AU - de Heer, K

AU - Meijer, M

AU - Janssen, H-G

AU - de Cock, H

AU - Wösten, H A B

AU - Visser, C.E.

AU - van Oers, M H J

AU - Sterk, P J

N1 - © The Author 2017. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: [email protected].

PY - 2018/2

Y1 - 2018/2

N2 - Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography-mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo.

AB - Volatile organic compounds (VOCs) in exhaled breath may identify the presence of invasive pulmonary aspergillosis. We aimed to detect VOC profiles emitted by in vitro cultured, clinical Aspergillus isolates using gas chromatography-mass spectrometry (GC-MS). Three clinical Aspergillus isolates and a reference strain were cultured while conidiation was prevented. Headspace samples were analyzed using a standardized method. Breath samples of patients from which the cultures were obtained were checked for the presence of the VOCs found in vitro. Each Aspergillus isolate produced a distinct VOC profile. These profiles could not be confirmed in exhaled breath in vivo.

KW - gas chromatography-mass spectrometry

KW - Aspergillus

KW - headspace analysis

KW - exhaled breath analysis

KW - invasive pulmonary aspergillosis

U2 - 10.1093/mmy/myx035

DO - 10.1093/mmy/myx035

M3 - Article

C2 - 28525576

SN - 1369-3786

VL - 56

SP - 253

EP - 256

JO - Medical Mycology

JF - Medical Mycology

IS - 2

ER -

Profiling of volatile organic compounds produced by clinical Aspergillus isolates using gas chromatography-mass spectrometry

Abstract

Keywords

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