Principles and applications of matrix-assisted UV-laser desorption/ionization mass spectrometry

M. Karas; U. Bahr; A. Ingendoh; E. Nordhoff; B. Stahl; K. Strupat; F. Hillenkamp

doi:10.1016/S0003-2670(00)83645-4

Principles and applications of matrix-assisted UV-laser desorption/ionization mass spectrometry

M. Karas^*, U. Bahr, A. Ingendoh, E. Nordhoff, B. Stahl, K. Strupat, F. Hillenkamp

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (LDI MS) has shown its potential for desorbing ions of biomolecules with relative molecular masses (M_r) up to about 300 000 daltons. Determinations of M_r with an accuracy of 0.01-0.2% from about 1 pmol of sample are possible. Examples are shown for proteins, glycoproteins, oligonucleotides and carbohydrates. Examples of the combination of LDI MS with biochemical methods are shown, including the determination of the carbohydrate content of glycoproteins, the identification of disulphide-bonded subunits by cleavage of the light and heavy chains of a monoclonal antibody, the detection of the time course of enzymatic reactions and the analysis of proteins bound to a PVDF membrane.

Original language	English
Pages (from-to)	175-185
Number of pages	11
Journal	Analytica Chimica Acta
Volume	241
Issue number	2
DOIs	https://doi.org/10.1016/S0003-2670(00)83645-4
Publication status	Published - 1990
Externally published	Yes

Keywords

Biological samples
Carbohydrates
Glycoproteins
Oligonucleotides
Proteins

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10.1016/S0003-2670(00)83645-4

Cite this

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title = "Principles and applications of matrix-assisted UV-laser desorption/ionization mass spectrometry",

abstract = "Matrix-assisted laser desorption/ionization mass spectrometry (LDI MS) has shown its potential for desorbing ions of biomolecules with relative molecular masses (Mr) up to about 300 000 daltons. Determinations of Mr with an accuracy of 0.01-0.2% from about 1 pmol of sample are possible. Examples are shown for proteins, glycoproteins, oligonucleotides and carbohydrates. Examples of the combination of LDI MS with biochemical methods are shown, including the determination of the carbohydrate content of glycoproteins, the identification of disulphide-bonded subunits by cleavage of the light and heavy chains of a monoclonal antibody, the detection of the time course of enzymatic reactions and the analysis of proteins bound to a PVDF membrane.",

keywords = "Biological samples, Carbohydrates, Glycoproteins, Oligonucleotides, Proteins",

author = "M. Karas and U. Bahr and A. Ingendoh and E. Nordhoff and B. Stahl and K. Strupat and F. Hillenkamp",

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TY - JOUR

T1 - Principles and applications of matrix-assisted UV-laser desorption/ionization mass spectrometry

AU - Karas, M.

AU - Bahr, U.

AU - Ingendoh, A.

AU - Nordhoff, E.

AU - Stahl, B.

AU - Strupat, K.

AU - Hillenkamp, F.

PY - 1990

Y1 - 1990

N2 - Matrix-assisted laser desorption/ionization mass spectrometry (LDI MS) has shown its potential for desorbing ions of biomolecules with relative molecular masses (Mr) up to about 300 000 daltons. Determinations of Mr with an accuracy of 0.01-0.2% from about 1 pmol of sample are possible. Examples are shown for proteins, glycoproteins, oligonucleotides and carbohydrates. Examples of the combination of LDI MS with biochemical methods are shown, including the determination of the carbohydrate content of glycoproteins, the identification of disulphide-bonded subunits by cleavage of the light and heavy chains of a monoclonal antibody, the detection of the time course of enzymatic reactions and the analysis of proteins bound to a PVDF membrane.

AB - Matrix-assisted laser desorption/ionization mass spectrometry (LDI MS) has shown its potential for desorbing ions of biomolecules with relative molecular masses (Mr) up to about 300 000 daltons. Determinations of Mr with an accuracy of 0.01-0.2% from about 1 pmol of sample are possible. Examples are shown for proteins, glycoproteins, oligonucleotides and carbohydrates. Examples of the combination of LDI MS with biochemical methods are shown, including the determination of the carbohydrate content of glycoproteins, the identification of disulphide-bonded subunits by cleavage of the light and heavy chains of a monoclonal antibody, the detection of the time course of enzymatic reactions and the analysis of proteins bound to a PVDF membrane.

KW - Biological samples

KW - Carbohydrates

KW - Glycoproteins

KW - Oligonucleotides

KW - Proteins

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DO - 10.1016/S0003-2670(00)83645-4

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JO - Analytica Chimica Acta

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Principles and applications of matrix-assisted UV-laser desorption/ionization mass spectrometry

Abstract

Keywords

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