Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells

Jasmijn V Korpershoek; Mylène de Ruijter; Bastiaan F Terhaard; Michella H Hagmeijer; Daniël B F Saris; Miguel Castilho; Jos Malda; Lucienne A Vonk

doi:10.3390/ijms222011200

Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells

Jasmijn V Korpershoek
, Mylène de Ruijter
, Bastiaan F Terhaard
, Michella H Hagmeijer
, Daniël B F Saris
, Miguel Castilho
, Jos Malda
, Lucienne A Vonk

Methodology & Statistics

Utrecht University, University Medical Center Utrecht and Rehabilitation Center

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young's modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment.

Original language	English
Article number	11200
Pages (from-to)	1-16
Journal	International Journal of Molecular Sciences
Volume	22
Issue number	20
DOIs	https://doi.org/10.3390/ijms222011200
Publication status	Published - 18 Oct 2021

Bibliographical note

Funding Information:
Funding: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research

Funding Information:
This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union?s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program ?Materials Driven Regeneration?, funded by the Netherlands Organization for Scientific Research (024.003.013).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

Keywords

Biofabrication
Clinical translation
Collagen meniscus implant®
Melt electrowriting
Meniscectomy18
Meniscus
Meniscus injury
Tissue-engineering

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Cite this

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title = "Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells",

abstract = "Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants{\textregistered} (CMI{\textregistered}). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI{\textregistered}, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young's modulus when compared to the CMI{\textregistered} scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment.",

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note = "Funding Information: Funding: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union{\textquoteright}s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research Funding Information: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union?s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program ?Materials Driven Regeneration?, funded by the Netherlands Organization for Scientific Research (024.003.013). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).",

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AU - Korpershoek, Jasmijn V

AU - Ruijter, Mylène de

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AU - Hagmeijer, Michella H

AU - Saris, Daniël B F

AU - Castilho, Miguel

AU - Malda, Jos

AU - Vonk, Lucienne A

N1 - Funding Information: Funding: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research Funding Information: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union?s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program ?Materials Driven Regeneration?, funded by the Netherlands Organization for Scientific Research (024.003.013). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

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N2 - Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young's modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment.

AB - Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young's modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment.

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DO - 10.3390/ijms222011200

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JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

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M1 - 11200

ER -

Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells

Abstract

Bibliographical note

Keywords

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