Polyunsaturated fatty acids alter the phenotype of human mast cells in vitro

L.W.J. Van Den Elsen; B. Schouten; M. Balvers; F.A. Redegeld; A.D. Kraneveld; E.F. Knol; J. Garssen; L.E.M. Willemsen

doi:10.1097/01.mpg.0000383075.98243.67

Abstract

Objectives and Study: The increased n-6:n-3 fatty acid ratio in Western diets may contribute to the rapid increase in prevalence of allergic diseases. Key effector cells in allergy are mast cells (MC). Methods: The effect of different fatty acids on MC activation was studied. Therefore separate long chain n-6 (arachidonic acid, AA) and n-3 (eicosapentaenoic acid, EPA and docosahexaenoic acid, DHA) polyunsaturated fatty acid (PUFA) incorporation was investigated in human MC lines (LAD2, HMC-1). Next to degranulation and mediator secretion (release of PGD2 and cytokines such as TNF-alpha, IL-4 and IL-13), generation of reactive oxygen species (ROS) and phosphorylation of mitogen-activated protein kinases (MAPK) was examined. Results: Incubation of MC with AA, EPA or DHA for 24 hours increased PUFA content of the cellular membrane. Incubation with PUFA did not reduce IgE-mediated degranulation by LAD2 cells. However, mediator release of ionomycin/PMA stimulated HMC-1 cells was differentially regulated. IL-13 (P

Original language	English
Pages (from-to)	12-13
Number of pages	2
Journal	Journal of Pediatric Gastroenterology and Nutrition
Volume	50
DOIs	https://doi.org/10.1097/01.mpg.0000383075.98243.67
Publication status	Published - 1 Jun 2010
Event	European Society for Paediatric Gastroenterology, Hepatology and Nutrition Annual Meeting - Istanbul, Turkey Duration: 9 Jun 2010 → 12 Jun 2010

Keywords

polyunsaturated fatty acid
interleukin 4
mitogen activated protein kinase
prostaglandin synthase inhibitor
fatty acid
arachidonic acid
icosapentaenoic acid
docosahexaenoic acid
cytokine
reactive oxygen metabolite
immunoglobulin E
omega 3 fatty acid
nutrition
in vitro study
mast cell
gastroenterology
human
society
phenotype
secretion (process)
allergic disease
degranulation
phosphorylation
prevalence
allergy
effector cell
cell membrane
mediator release
diet
cell activation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1097/01.mpg.0000383075.98243.67

Cite this

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title = "Polyunsaturated fatty acids alter the phenotype of human mast cells in vitro",

abstract = "Objectives and Study: The increased n-6:n-3 fatty acid ratio in Western diets may contribute to the rapid increase in prevalence of allergic diseases. Key effector cells in allergy are mast cells (MC). Methods: The effect of different fatty acids on MC activation was studied. Therefore separate long chain n-6 (arachidonic acid, AA) and n-3 (eicosapentaenoic acid, EPA and docosahexaenoic acid, DHA) polyunsaturated fatty acid (PUFA) incorporation was investigated in human MC lines (LAD2, HMC-1). Next to degranulation and mediator secretion (release of PGD2 and cytokines such as TNF-alpha, IL-4 and IL-13), generation of reactive oxygen species (ROS) and phosphorylation of mitogen-activated protein kinases (MAPK) was examined. Results: Incubation of MC with AA, EPA or DHA for 24 hours increased PUFA content of the cellular membrane. Incubation with PUFA did not reduce IgE-mediated degranulation by LAD2 cells. However, mediator release of ionomycin/PMA stimulated HMC-1 cells was differentially regulated. IL-13 (P",

keywords = "polyunsaturated fatty acid, interleukin 4, mitogen activated protein kinase, prostaglandin synthase inhibitor, fatty acid, arachidonic acid, icosapentaenoic acid, docosahexaenoic acid, cytokine, reactive oxygen metabolite, immunoglobulin E, omega 3 fatty acid, nutrition, in vitro study, mast cell, gastroenterology, human, society, phenotype, secretion (process), allergic disease, degranulation, phosphorylation, prevalence, allergy, effector cell, cell membrane, mediator release, diet, cell activation",

author = "{Van Den Elsen}, L.W.J. and B. Schouten and M. Balvers and F.A. Redegeld and A.D. Kraneveld and E.F. Knol and J. Garssen and L.E.M. Willemsen",

year = "2010",

month = jun,

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doi = "10.1097/01.mpg.0000383075.98243.67",

language = "English",

volume = "50",

pages = "12--13",

journal = "Journal of Pediatric Gastroenterology and Nutrition",

issn = "0277-2116",

publisher = "John Wiley and Sons Inc.",

note = "European Society for Paediatric Gastroenterology, Hepatology and Nutrition Annual Meeting ; Conference date: 09-06-2010 Through 12-06-2010",

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TY - JOUR

T1 - Polyunsaturated fatty acids alter the phenotype of human mast cells in vitro

AU - Van Den Elsen, L.W.J.

AU - Schouten, B.

AU - Balvers, M.

AU - Redegeld, F.A.

AU - Kraneveld, A.D.

AU - Knol, E.F.

AU - Garssen, J.

AU - Willemsen, L.E.M.

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Objectives and Study: The increased n-6:n-3 fatty acid ratio in Western diets may contribute to the rapid increase in prevalence of allergic diseases. Key effector cells in allergy are mast cells (MC). Methods: The effect of different fatty acids on MC activation was studied. Therefore separate long chain n-6 (arachidonic acid, AA) and n-3 (eicosapentaenoic acid, EPA and docosahexaenoic acid, DHA) polyunsaturated fatty acid (PUFA) incorporation was investigated in human MC lines (LAD2, HMC-1). Next to degranulation and mediator secretion (release of PGD2 and cytokines such as TNF-alpha, IL-4 and IL-13), generation of reactive oxygen species (ROS) and phosphorylation of mitogen-activated protein kinases (MAPK) was examined. Results: Incubation of MC with AA, EPA or DHA for 24 hours increased PUFA content of the cellular membrane. Incubation with PUFA did not reduce IgE-mediated degranulation by LAD2 cells. However, mediator release of ionomycin/PMA stimulated HMC-1 cells was differentially regulated. IL-13 (P

AB - Objectives and Study: The increased n-6:n-3 fatty acid ratio in Western diets may contribute to the rapid increase in prevalence of allergic diseases. Key effector cells in allergy are mast cells (MC). Methods: The effect of different fatty acids on MC activation was studied. Therefore separate long chain n-6 (arachidonic acid, AA) and n-3 (eicosapentaenoic acid, EPA and docosahexaenoic acid, DHA) polyunsaturated fatty acid (PUFA) incorporation was investigated in human MC lines (LAD2, HMC-1). Next to degranulation and mediator secretion (release of PGD2 and cytokines such as TNF-alpha, IL-4 and IL-13), generation of reactive oxygen species (ROS) and phosphorylation of mitogen-activated protein kinases (MAPK) was examined. Results: Incubation of MC with AA, EPA or DHA for 24 hours increased PUFA content of the cellular membrane. Incubation with PUFA did not reduce IgE-mediated degranulation by LAD2 cells. However, mediator release of ionomycin/PMA stimulated HMC-1 cells was differentially regulated. IL-13 (P

KW - polyunsaturated fatty acid

KW - interleukin 4

KW - mitogen activated protein kinase

KW - prostaglandin synthase inhibitor

KW - fatty acid

KW - arachidonic acid

KW - icosapentaenoic acid

KW - docosahexaenoic acid

KW - cytokine

KW - reactive oxygen metabolite

KW - immunoglobulin E

KW - omega 3 fatty acid

KW - nutrition

KW - in vitro study

KW - mast cell

KW - gastroenterology

KW - human

KW - society

KW - phenotype

KW - secretion (process)

KW - allergic disease

KW - degranulation

KW - phosphorylation

KW - prevalence

KW - allergy

KW - effector cell

KW - cell membrane

KW - mediator release

KW - diet

KW - cell activation

U2 - 10.1097/01.mpg.0000383075.98243.67

DO - 10.1097/01.mpg.0000383075.98243.67

M3 - Meeting Abstract

SN - 0277-2116

VL - 50

SP - 12

EP - 13

JO - Journal of Pediatric Gastroenterology and Nutrition

JF - Journal of Pediatric Gastroenterology and Nutrition

T2 - European Society for Paediatric Gastroenterology, Hepatology and Nutrition Annual Meeting

Y2 - 9 June 2010 through 12 June 2010