Plasmid Mediated Streptomycin and Sulfonamide Resistance in Haemophilus parasuis

  • Pin Chen
  • , Yingyu Liu
  • , Yang Wang
  • , Wentao Li
  • , Dingren Bi
  • , Qigai He*
  • *Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Streptomycin and sulfonamide resistance in Haemophilus parasuis is an emerging phenomenon that has not yet been characterized from a molecular perspective. The aim of the present research was to determine the molecular mechanism of Haemophilus parasuis resistant to streptomycin and sulfonamide. One high level streptomycin and sulfonamide-resistant isolate from China was submitted for MIC determination and plasm:id extract, then sequencing and annotation. One plasmid extracted from the bacteria strain, designated pHPS-A67 (4.2 kb), contain fifteen open reading frames including streptomycin resistance gene StrA, sulfonamide resistance gene Sul 2 and mobilization protein genes Mob A, Mob B and Mob C. This is believed to be the first report of native Haemophilus parasuis contains plasmid which mediated streptomycin and sulfonamide resistance in this microorganism.

    Original languageEnglish
    Pages (from-to)1106-1109
    Number of pages4
    JournalJournal of Animal and Veterinary Advances
    Volume11
    Issue number8
    Publication statusPublished - 2012

    Funding

    This research was supported by the earmarked fund for China Agriculture Research System (CARS-36). The researchers wish to thank Xueli Zhou for his kind donation of the Haemophilus parasuis isolated strain which used for plasmid isolation.

    Keywords

    • Haemophilus parasuis
    • streptomycin
    • sulfonamide
    • plasmid
    • pig
    • China
    • SEQUENCE

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