Abstract
Phospholipids are believed to play an important role in pathology and physiology of the myocardium. Because of the distinct physico-chemical properties of plasmalogens we studied the plasmalogen content and distribution in the sarcolemma of cultured rat myocytes. Treatment with phospholipase A2 degraded all glycerophospholipids in the outer monolayer. The hydrolysis products were analyzed for plasmalogen content. It is shown that the inner sarcolemmal leaflet is highly enriched in phosphatidylcholine and ethanolamine plasmalogen. This distribution of the plasmalogens might affect bilayer stability and thereby be involved in the destruction of the sarcolemma upon ischemia and reperfusion.
| Original language | English |
|---|---|
| Pages (from-to) | 78-82 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 240 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 21 Nov 1988 |
Keywords
- (Cultured myocyte
- Neonatal rat heart)
- Phospholipase
- Phospholipid asymmetry
- Plasma membrane
- Plasmalogen
- animal cell
- animal experiment
- animal model
- bilayer membrane
- destruction
- hydrolysis
- ischemia
- muscle fiber culture
- newborn
- nonhuman
- physicochemical model
- reperfusion
- sarcolemma
- phosphatidylcholine
- phospholipase A2
- plasmalogen