@article{305b65114376448ab84c25b68edabaa9,
title = "Plasma and urinary metanephrine and normetanephrine concentrations using liquid chromatography with tandem mass spectrometry in healthy cats and in a cat with pheochromocytoma",
abstract = "BACKGROUND: Pheochromocytoma (PCC) is rare in cats and plasma (PL) and urinary (U) metanephrines (metanephrine [MN]; normetanephrine [NMN]) measurement is rarely described in cats.OBJECTIVES: We evaluated the utility of PL and U MNs measurement in 10 healthy cats and a cat with a confirmed diagnosis of pheochromocytoma (PheoCat), using liquid chromatography with tandem mass spectrometry (LC-MS-MS).METHODS: Urine and EDTA PL samples collected from each of the 10 cats and the PheoCat were promptly stored at -80°C and remained frozen until analysis. To evaluate U MNs stability, an additional urine sample collected from the healthy cats was refrigerated for 24 hours before freezing. Urinary creatinine concentration (Creat) was assessed using the same spot urine samples to calculate U MNs-to-creatinine ratios.RESULTS: The PL-MN and PL-NMN median concentrations of the healthy cats were 2.73 and 7.02 nmol/L, respectively. The median U-MN/Creat and U-NMN/Creat ratios were 70 and 139 μg/g, respectively. The PheoCat had a PL-MN of 3.68 nmol/L, PL-NMN of 66.27 nmol/L, U-MN/Creat of 179 μg/g, and U-NMN/Creat of 1262 μg/g. The PheoCat had markedly increased concentrations of both PL and U MNs when compared to the healthy cats. No significant difference was found between U MNs measured in urine samples that underwent 24 hours of refrigeration in comparison to those that were frozen immediately.CONCLUSIONS: We report preliminary reference intervals for PL and U MNs in cats using LC-MS-MS and the potential clinical applicability of these biomarkers for the diagnosis of PCC in cats.",
keywords = "feline pheochromocytoma, healthy cats, liquid chromatography with tandem mass spectrometry, plasma and urinary metanephrines",
author = "Prego, {Maria Teresa} and Dias, {Maria Joana} and Ferreira, {Rui Lemos} and Salom{\'e} Gon{\c c}alves and {Dias Domingues}, Tiago and Greet Junius and {Van den Steen}, Evi and Sara Galac and Leal, {Rodolfo Oliveira}",
note = "Funding Information: This work was supported by FCT—Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia IP, grant UIDB/00276/2020 and by LA/P/0059/2020—AL4AnimalS. Part of this work was presented as an oral abstract at the European College of Veterinary Internal Medicine—Companion Animals Annual 32nd Congress 2022. Authors thank Centro de Investiga{\c c}{\~a}o Interdisciplinar em Sanidade Animal for the support and funding of this study, and students and staff from the Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Lisbon for actively participating in this project. Authors also thank the Algemeen Medisch Laboratorium for the technical support provided. Funding Information: This work was supported by FCT—Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia IP, grant UIDB/00276/2020 and by LA/P/0059/2020—AL4AnimalS. Part of this work was presented as an oral abstract at the European College of Veterinary Internal Medicine—Companion Animals Annual 32nd Congress 2022. Authors thank Centro de Investiga{\c c}{\~a}o Interdisciplinar em Sanidade Animal for the support and funding of this study, and students and staff from the Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Lisbon for actively participating in this project. Authors also thank the Algemeen Medisch Laboratorium for the technical support provided. Publisher Copyright: {\textcopyright} 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.",
year = "2023",
month = may,
day = "1",
doi = "10.1111/jvim.16648",
language = "English",
volume = "37",
pages = "910--914",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley-Blackwell",
number = "3",
}