Physical and linkage mapping of the bovine acidic alpha-glucosidase gene to chromosome 19

Physical and linkage mapping of the bovine acidic a-glucosidase gene to chromosome 19 I Tammen1, U Larsson1,5, N Bergknut1,5, W Barendse2, C Moran3, J A Dennis4 1 ReproGen, Centre for Advanced Technologies in Animal Genetics and Reproduction, Department of Veterinary Clinical Sciences, The University of Sydney, Camden, NSW 2570, Australia; 2 CSIRO, Tropical Agriculture, Gehrman Laboratories, University of Queensland, St Lucia 4072, Australia; 3 Department of Animal Science, University of Sydney, Sydney, NSW 2006, Australia; 4 Elizabeth Macarthur Agricultural Institute, NSW Agriculture, Menangle, NSW 2568, Australia; Present address: 5 Swedish University of Agricultural Sciences, 75124 Uppsala, Sweden. Accepted 31 March 2000 Description: PCR primers were selected from the bovine sequence for the acidic a-glucosidase gene1 (GAA) (GenBank accession no. AF171666). Two pairs of primers were chosen from exon 2:intron 2 (GAA-E2 and GAA-I2) and exon 9:intron 9 (GAA-E9 and GAA-I9) for mapping on a bovine somatic cell hybrid panel2. At least one intronic primer was chosen to ensure bovine specificity. For linkage mapping on the international bovine reference panel (IBRP)3, primers GAA-E14 and GAA-E15, which flank a silent mutation in exon 15 (C2025T)1, were selected.