Photopolymerized thermosensitive hydrogels for tailorable diffusion-controlled protein delivery.

R. Censi, T. Vermonden, M.J. van Steenbergen, H. Deschout, K. Braeckmans, S.C. de Smedt, C.F. van Nostrum, P. di Martino, W.E. Hennink

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In this paper the possibility to tailor degradation and protein release behavior of photopolymerized thermosensitive hydrogels is studied. The hydrogels consist of ABA triblock copolymer, in which the thermosensitive A-blocks are methacrylated poly(N-(2-hydroxypropyl)methacrylamide lactate)s and the B-block is poly(ethylene glycol) with molecular weight of 10 kDa. These hydrogels are prepared by using a combination of physical and chemical cross-linking methods. When a solution of a thermosensitive methacrylated p(HPMAm-lac)-PEG-p(HPMAm-lac) is heated above its cloud point a viscoelastic material is obtained, which can be stabilized by introducing covalent cross-links by photopolymerization. By varying the polymer concentration, hydrogels with different mechanical properties are formed, of which the cross-linking density, mesh size, swelling and degradation behavior can be tuned. It was demonstrated that the release rate of three model proteins (lysozyme, BSA and IgG, with hydrodynamic diameters ranging from 4.1 to 10.7 nm) depended on the protein size and hydrogel molecular weight between cross-links and was governed by the Fickian diffusion. Importantly, the encapsulated proteins were quantitatively released and the secondary structure and the enzymatic activity of lysozyme were fully preserved demonstrating the protein friendly nature of the studied delivery system.
Original languageUndefined/Unknown
Pages (from-to)230-6
Number of pages7
JournalJournal of Controlled Release
Volume140
Issue number3
Publication statusPublished - 2009

Keywords

  • Farmacie/Biofarmaceutische wetenschappen (FARM)
  • Medical technology
  • Farmacie(FARM)
  • Biomedische technologie en medicijnen
  • Pharmacology

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