Abstract
We show that a comprehensive set of 16 peroxisomal membrane proteins (PMPs) encompassing all types of membrane
topologies first target to the endoplasmic reticulum (ER) in Saccharomyces cerevisiae. These PMPs insert into the ER
membrane via the protein import complexes Sec61p and Get3p (for tail-anchored proteins). This trafficking pathway is
representative for multiplying wild-type cells in which the peroxisome population needs to be maintained, as well as for
mutant cells lacking peroxisomes in which new peroxisomes form after complementation with the wild-type version of
the mutant gene. PMPs leave the ER in a Pex3p-Pex19p–dependent manner to end up in metabolically active peroxisomes.
These results further extend the new concept that peroxisomes derive their basic framework (membrane and membrane
proteins) from the ER and imply a new functional role for Pex3p and Pex19p.
Original language | English |
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Pages (from-to) | 2057-2065 |
Number of pages | 9 |
Journal | Molecular Biology of the Cell |
Volume | 21 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2010 |