Perdeuterated and 13C-enriched myo-inositol for DNP assisted monitoring of enzymatic phosphorylation by inositol-3-kinase

M J Moure; Y Zhuo; G J Boons; James H Prestegard

doi:10.1039/c7cc07023c

Perdeuterated and 13C-enriched myo-inositol for DNP assisted monitoring of enzymatic phosphorylation by inositol-3-kinase

M J Moure, Y Zhuo, G J Boons, James H Prestegard

Chemical Biology and Drug Discovery

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The synthesis of perdeuterated and 13C enriched myo-inositol is presented. Myo-inositol and its derivatives are of interest as substrates for enzymes producing phosphorylated species with regulatory functions in many organisms. Its utility in monitoring real-time phosphorylation by myo-inositol-3-kinase is illustrated using dynamic nuclear polarization (DNP) to enhance NMR observation.

Original language	English
Pages (from-to)	12398-12401
Number of pages	4
Journal	Chemical Communications
Volume	53
Issue number	92
DOIs	https://doi.org/10.1039/c7cc07023c
Publication status	Published - 16 Nov 2017

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title = "Perdeuterated and 13C-enriched myo-inositol for DNP assisted monitoring of enzymatic phosphorylation by inositol-3-kinase",

abstract = "The synthesis of perdeuterated and 13C enriched myo-inositol is presented. Myo-inositol and its derivatives are of interest as substrates for enzymes producing phosphorylated species with regulatory functions in many organisms. Its utility in monitoring real-time phosphorylation by myo-inositol-3-kinase is illustrated using dynamic nuclear polarization (DNP) to enhance NMR observation.",

author = "Moure, {M J} and Y Zhuo and Boons, {G J} and Prestegard, {James H}",

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AU - Moure, M J

AU - Zhuo, Y

AU - Boons, G J

AU - Prestegard, James H

PY - 2017/11/16

Y1 - 2017/11/16

N2 - The synthesis of perdeuterated and 13C enriched myo-inositol is presented. Myo-inositol and its derivatives are of interest as substrates for enzymes producing phosphorylated species with regulatory functions in many organisms. Its utility in monitoring real-time phosphorylation by myo-inositol-3-kinase is illustrated using dynamic nuclear polarization (DNP) to enhance NMR observation.

AB - The synthesis of perdeuterated and 13C enriched myo-inositol is presented. Myo-inositol and its derivatives are of interest as substrates for enzymes producing phosphorylated species with regulatory functions in many organisms. Its utility in monitoring real-time phosphorylation by myo-inositol-3-kinase is illustrated using dynamic nuclear polarization (DNP) to enhance NMR observation.

U2 - 10.1039/c7cc07023c

DO - 10.1039/c7cc07023c

M3 - Article

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VL - 53

SP - 12398

EP - 12401

JO - Chemical Communications

JF - Chemical Communications

IS - 92

ER -

Perdeuterated and 13C-enriched myo-inositol for DNP assisted monitoring of enzymatic phosphorylation by inositol-3-kinase

Abstract

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