Abstract
How steroid hormone receptors (SHRs) regulate transcriptional activity remains partly understood. Upon activation, SHRs bind the genome together with a co-regulator repertoire, crucial to induce gene expression. However, it remains unknown which components of the SHR-recruited co-regulator complex are essential to drive transcription following hormonal stimuli. Through a FACS-based genome-wide CRISPR screen, we functionally dissected the Glucocorticoid Receptor (GR) complex. We describe a functional cross-talk between PAXIP1 and the cohesin subunit STAG2, critical for regulation of gene expression by GR. Without altering the GR cistrome, PAXIP1 and STAG2 depletion alter the GR transcriptome, by impairing the recruitment of 3D-genome organization proteins to the GR complex. Importantly, we demonstrate that PAXIP1 is required for stability of cohesin on chromatin, its localization to GR-occupied sites, and maintenance of enhancer-promoter interactions. In lung cancer, where GR acts as tumor suppressor, PAXIP1/STAG2 loss enhances GR-mediated tumor suppressor activity by modifying local chromatin interactions. All together, we introduce PAXIP1 and STAG2 as novel co-regulators of GR, required to maintain 3D-genome architecture and drive the GR transcriptional programme following hormonal stimuli.
Original language | English |
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Pages (from-to) | 9576-9593 |
Number of pages | 18 |
Journal | Nucleic Acids Research |
Volume | 51 |
Issue number | 18 |
DOIs | |
Publication status | Published - 13 Oct 2023 |
Bibliographical note
Publisher Copyright:© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.
Funding
We thank members of the Zwart and Bergman labs for valuable feedback, suggestions and input. This work was supported by KWF/Alpe d’Huzes and Oncode Institute. We would like to thank the NKI genomics core facility for next-generation sequencing and bioinformatics support. We thank the NKI Flow Cytometry facility for technical support. We thank the NKI Proteomics/Mass Spectrometry facility (M.A. and L.H. are supported by the Dutch NWO X-omics Initiative). We thank Reuven Agami, Julien Champagne and Remco Nagel for fruitful discussions and technical support. KWF Kankerbestrijding/Alpe d’Huzes; Oncode Institute; Dutch NWO X-omics Initiative; NWO Building Blocks of Life program project Genometrack (737.016.014). Funding for open access charge: Financial resources are available to cover publication charges. We thank members of the Zwart and Bergman labs for valuable feedback, suggestions and input. This work was supported by KWF/Alpe d’Huzes and Oncode Institute. We would like to thank the NKI genomics core facility for next-generation sequencing and bioinformatics support. We thank the NKI Flow Cytometry facility for technical support. We thank the NKI Proteomics/Mass Spectrometry facility (M.A. and L.H. are supported by the Dutch NWO X-omics Initiative). We thank Reuven Agami, Julien Champagne and Remco Nagel for fruitful discussions and technical support.
Funders | Funder number |
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Alpe d’Huzes | |
NWO X-omics Initiative | |
Reuven Agami | |
Nederlandse Organisatie voor Wetenschappelijk Onderzoek | 737.016.014 |
KWF Kankerbestrijding | |
Oncode Institute |