Abstract
The influence of the leukotoxin of Pasteurella haemolytica on the generation of arachidonic acid metabolites by bovine polymorphonuclear leukocytes (PMNs) was investigated. PMNs released 5-, 12-, and 15- hydroxyeicosatetraenoic acids (5-, 12-, and 15-HETE) and leukotriene B4(LTB4) upon stimulation with arachidonic acid. The leukotoxin preparations dose dependently enhanced the release of the 5-lipoxygenase products 5-HETE and LTB4in arachidonic acid-stimulated PMNs, whereas the release of 12- and 15-HETE was not affected. The enhanced release of LTB4and 5-HETE was not due to a decreased cellular retention of the 5-lipoxygenase products. In addition, leukotoxin preparations by themselves were also able to induce LTB4and 5-HETE production in the absence of exogenous arachidonic acid. Generation of 5-lipoxygenase products by PMNs stimulated by leukotoxin may represent an important cellular event that occurs during infections with P. haemolytica.
| Original language | English |
|---|---|
| Pages (from-to) | 3238-3243 |
| Number of pages | 6 |
| Journal | Infection and Immunity |
| Volume | 60 |
| Issue number | 8 |
| Publication status | Published - 26 Jan 1992 |
Keywords
- 12 hydroxyicosatetraenoic acid
- 15 hydroxyicosatetraenoic acid
- 5 hydroxyicosatetraenoic acid
- arachidonic acid
- leukotoxin
- leukotriene B4
- animal cell
- arachidonic acid metabolism
- article
- cow
- dose response
- Gram negative infection
- human
- human cell
- immunostimulation
- inflammation
- inflammatory disease
- leukocyte metabolism
- Mannheimia haemolytica
- neutrophil
- neutrophil chemotaxis
- nonhuman
- normal human
- priority journal