Pandemic H1N1 vaccine requires the use of an adjuvant to protect against challenge in naïve ferrets

Benoît Baras; Leon de Waal; Koert J Stittelaar; Valérie Jacob; Sandra Giannini; Edwin J B Veldhuis Kroeze; Judith M A van den Brand; Geert van Amerongen; James H Simon; Emmanuel Hanon; Sally P Mossman; Albert D M E Osterhaus

doi:10.1016/j.vaccine.2010.12.125

Pandemic H1N1 vaccine requires the use of an adjuvant to protect against challenge in naïve ferrets

Benoît Baras
, Leon de Waal
, Koert J Stittelaar
, Valérie Jacob
, Sandra Giannini
, Edwin J B Veldhuis Kroeze
, Judith M A van den Brand
, Geert van Amerongen
, James H Simon
, Emmanuel Hanon
, Sally P Mossman
, Albert D M E Osterhaus

GlaxoSmithKline Biologicals, Rixensart, Belgium. [email protected]

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In the context of an A/H1N1 influenza pandemic situation, this study demonstrates that heterologous vaccination with an AS03-adjuvanted 2008/2009 seasonal trivalent and pandemic H5N1 monovalent split vaccine conferred partial protection in influenza-naïve ferrets after challenge with the influenza pandemic H1N1 A/The Netherlands/602/09 virus. Further, unlike saline control and non-adjuvanted vaccine, it was shown that immunization of naïve ferrets with an AS03-adjuvanted pandemic H1N1 A/California/7/09 influenza split vaccine induced increased antibody response and enhanced protection against the challenge strain, including significant reduction in viral shedding in the upper respiratory tract and reduced lung pathology post-challenge. These results show the need for vaccination with the adjuvanted vaccine to fully protect against viral replication and influenza disease in unprimed ferrets.

Original language	English
Pages (from-to)	2120-6
Number of pages	7
Journal	Vaccine
Volume	29
Issue number	11
DOIs	https://doi.org/10.1016/j.vaccine.2010.12.125
Publication status	Published - 2011

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Adjuvants, Immunologic
Animals
Antibodies, Viral
Ferrets
Influenza A Virus, H1N1 Subtype
Influenza A Virus, H5N1 Subtype
Influenza Vaccines
Lung
Neutralization Tests
Orthomyxoviridae Infections
Viral Load
Virus Replication
Virus Shedding
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Access to Document

10.1016/j.vaccine.2010.12.125

Cite this

Baras, B., de Waal, L., Stittelaar, K. J., Jacob, V., Giannini, S., Kroeze, E. J. B. V., van den Brand, J. M. A., Amerongen, G. V., Simon, J. H., Hanon, E., Mossman, S. P., & Osterhaus, A. D. M. E. (2011). Pandemic H1N1 vaccine requires the use of an adjuvant to protect against challenge in naïve ferrets. Vaccine, 29(11), 2120-6. https://doi.org/10.1016/j.vaccine.2010.12.125

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title = "Pandemic H1N1 vaccine requires the use of an adjuvant to protect against challenge in na{\"i}ve ferrets",

abstract = "In the context of an A/H1N1 influenza pandemic situation, this study demonstrates that heterologous vaccination with an AS03-adjuvanted 2008/2009 seasonal trivalent and pandemic H5N1 monovalent split vaccine conferred partial protection in influenza-na{\"i}ve ferrets after challenge with the influenza pandemic H1N1 A/The Netherlands/602/09 virus. Further, unlike saline control and non-adjuvanted vaccine, it was shown that immunization of na{\"i}ve ferrets with an AS03-adjuvanted pandemic H1N1 A/California/7/09 influenza split vaccine induced increased antibody response and enhanced protection against the challenge strain, including significant reduction in viral shedding in the upper respiratory tract and reduced lung pathology post-challenge. These results show the need for vaccination with the adjuvanted vaccine to fully protect against viral replication and influenza disease in unprimed ferrets.",

keywords = "Adjuvants, Immunologic, Animals, Antibodies, Viral, Ferrets, Influenza A Virus, H1N1 Subtype, Influenza A Virus, H5N1 Subtype, Influenza Vaccines, Lung, Neutralization Tests, Orthomyxoviridae Infections, Viral Load, Virus Replication, Virus Shedding, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",

author = "Beno{\^i}t Baras and \{de Waal\}, Leon and Stittelaar, \{Koert J\} and Val{\'e}rie Jacob and Sandra Giannini and Kroeze, \{Edwin J B Veldhuis\} and \{van den Brand\}, \{Judith M A\} and Amerongen, \{Geert van\} and Simon, \{James H\} and Emmanuel Hanon and Mossman, \{Sally P\} and Osterhaus, \{Albert D M E\}",

year = "2011",

doi = "10.1016/j.vaccine.2010.12.125",

language = "English",

volume = "29",

pages = "2120--6",

journal = "Vaccine",

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AU - Baras, Benoît

AU - de Waal, Leon

AU - Stittelaar, Koert J

AU - Jacob, Valérie

AU - Giannini, Sandra

AU - Kroeze, Edwin J B Veldhuis

AU - van den Brand, Judith M A

AU - Amerongen, Geert van

AU - Simon, James H

AU - Hanon, Emmanuel

AU - Mossman, Sally P

AU - Osterhaus, Albert D M E

PY - 2011

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N2 - In the context of an A/H1N1 influenza pandemic situation, this study demonstrates that heterologous vaccination with an AS03-adjuvanted 2008/2009 seasonal trivalent and pandemic H5N1 monovalent split vaccine conferred partial protection in influenza-naïve ferrets after challenge with the influenza pandemic H1N1 A/The Netherlands/602/09 virus. Further, unlike saline control and non-adjuvanted vaccine, it was shown that immunization of naïve ferrets with an AS03-adjuvanted pandemic H1N1 A/California/7/09 influenza split vaccine induced increased antibody response and enhanced protection against the challenge strain, including significant reduction in viral shedding in the upper respiratory tract and reduced lung pathology post-challenge. These results show the need for vaccination with the adjuvanted vaccine to fully protect against viral replication and influenza disease in unprimed ferrets.

AB - In the context of an A/H1N1 influenza pandemic situation, this study demonstrates that heterologous vaccination with an AS03-adjuvanted 2008/2009 seasonal trivalent and pandemic H5N1 monovalent split vaccine conferred partial protection in influenza-naïve ferrets after challenge with the influenza pandemic H1N1 A/The Netherlands/602/09 virus. Further, unlike saline control and non-adjuvanted vaccine, it was shown that immunization of naïve ferrets with an AS03-adjuvanted pandemic H1N1 A/California/7/09 influenza split vaccine induced increased antibody response and enhanced protection against the challenge strain, including significant reduction in viral shedding in the upper respiratory tract and reduced lung pathology post-challenge. These results show the need for vaccination with the adjuvanted vaccine to fully protect against viral replication and influenza disease in unprimed ferrets.

KW - Adjuvants, Immunologic

KW - Animals

KW - Antibodies, Viral

KW - Ferrets

KW - Influenza A Virus, H1N1 Subtype

KW - Influenza A Virus, H5N1 Subtype

KW - Influenza Vaccines

KW - Lung

KW - Neutralization Tests

KW - Orthomyxoviridae Infections

KW - Viral Load

KW - Virus Replication

KW - Virus Shedding

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.vaccine.2010.12.125

DO - 10.1016/j.vaccine.2010.12.125

M3 - Article

C2 - 21238573

SN - 0264-410X

VL - 29

SP - 2120

EP - 2126

JO - Vaccine

JF - Vaccine

IS - 11

ER -

Pandemic H1N1 vaccine requires the use of an adjuvant to protect against challenge in naïve ferrets

Abstract

UN SDGs

Keywords

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