TY - JOUR
T1 - P292 10 Hz rTMS induces long-term depression of GABAergic neurotransmission
AU - Lenz, Maximilian
AU - Galanis, Christos
AU - Müller-Dahlhaus, Florian
AU - Opitz, Alexander
AU - Wierenga, C.J.
AU - Szabó, Gábor
AU - Ziemann, Ulf
AU - Deller, T.
AU - Funke, Klaus
AU - Vlachos, Andreas
PY - 2017/3
Y1 - 2017/3
N2 - Question The cellular and molecular mechanisms of rTMS-induced neural plasticity remain not well understood. Recent experimental evidence obtained in mouse brain slice cultures disclosed that 10 Hz repetitive magnetic stimulation (rMS) induces long-term potentiation (LTP) of excitatory neurotransmission. These studies support the notion that rTMS acts through the induction of ‘LTP-like’ plasticity. Here, we tested whether rTMS increases cortical excitability by mediating long-term depression (LTD) of inhibitory neurotransmission. Methods Immunohistochemistry, fluorescence recovery after photobleaching, GABA-uncaging experiments and paired whole-cell patch clamp recordings were used in entorhino-hippocampal slice cultures to study the effects of 10 Hz rMS on structural and functional properties of inhibitory synapses of CA1 pyramidal neurons. Results We report that 10 Hz rMS reduces dendritic but not somatic GABAergic neurotransmission onto CA1 pyramidal neurons. These functional changes are accompanied by structural remodeling of inhibitory postsynapses and depend on the activation of voltage gated sodium channels, L-type voltage gated calcium channels, NMDA-receptors, and calcineurin protein phosphatases. Consistent with these findings, a reduction in gephyrin cluster sizes and numbers is detected in CA1 stratum radiatum 2 h after 10 Hz rTMS of anaesthetized mice. Conclusion rMS appears to be a potent tool to induce Ca 2+ -dependent structural and functional changes of both excitatory and inhibitory synapses. We propose that 10 Hz rTMS may shift excitation/inhibition-balance in neuronal networks by inducing LTP of excitatory and LTD of inhibitory neurotransmission. (Supported by DFG and Federal Ministry of Education and Research, Germany; GCBS-WP1.) [ABSTRACT FROM AUTHOR] Copyright of Clinical Neurophysiology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
AB - Question The cellular and molecular mechanisms of rTMS-induced neural plasticity remain not well understood. Recent experimental evidence obtained in mouse brain slice cultures disclosed that 10 Hz repetitive magnetic stimulation (rMS) induces long-term potentiation (LTP) of excitatory neurotransmission. These studies support the notion that rTMS acts through the induction of ‘LTP-like’ plasticity. Here, we tested whether rTMS increases cortical excitability by mediating long-term depression (LTD) of inhibitory neurotransmission. Methods Immunohistochemistry, fluorescence recovery after photobleaching, GABA-uncaging experiments and paired whole-cell patch clamp recordings were used in entorhino-hippocampal slice cultures to study the effects of 10 Hz rMS on structural and functional properties of inhibitory synapses of CA1 pyramidal neurons. Results We report that 10 Hz rMS reduces dendritic but not somatic GABAergic neurotransmission onto CA1 pyramidal neurons. These functional changes are accompanied by structural remodeling of inhibitory postsynapses and depend on the activation of voltage gated sodium channels, L-type voltage gated calcium channels, NMDA-receptors, and calcineurin protein phosphatases. Consistent with these findings, a reduction in gephyrin cluster sizes and numbers is detected in CA1 stratum radiatum 2 h after 10 Hz rTMS of anaesthetized mice. Conclusion rMS appears to be a potent tool to induce Ca 2+ -dependent structural and functional changes of both excitatory and inhibitory synapses. We propose that 10 Hz rTMS may shift excitation/inhibition-balance in neuronal networks by inducing LTP of excitatory and LTD of inhibitory neurotransmission. (Supported by DFG and Federal Ministry of Education and Research, Germany; GCBS-WP1.) [ABSTRACT FROM AUTHOR] Copyright of Clinical Neurophysiology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
U2 - 10.1016/j.clinph.2016.10.400
DO - 10.1016/j.clinph.2016.10.400
M3 - Meeting Abstract
C2 - 121275979
SN - 1388-2457
VL - 128
SP - e152-e153
JO - Clinical Neurophysiology
JF - Clinical Neurophysiology
IS - 3
ER -