@article{e0d0470ed44244e5bb26f77656900b33,
title = "Optical Tweezers Approaches for Probing Multiscale Protein Mechanics and Assembly",
abstract = "Multi-step assembly of individual protein building blocks is key to the formation of essential higher-order structures inside and outside of cells. Optical tweezers is a technique well suited to investigate the mechanics and dynamics of these structures at a variety of size scales. In this mini-review, we highlight experiments that have used optical tweezers to investigate protein assembly and mechanics, with a focus on the extracellular matrix protein collagen. These examples demonstrate how optical tweezers can be used to study mechanics across length scales, ranging from the single-molecule level to fibrils to protein networks. We discuss challenges in experimental design and interpretation, opportunities for integration with other experimental modalities, and applications of optical tweezers to current questions in protein mechanics and assembly.",
keywords = "collagen, fibrillar proteins, microrheology, optical tweezers (OT), protein assemblies, protein mechanics, protein structure/folding, single molecule",
author = "Kathrin Lehmann and Marjan Shayegan and Blab, {Gerhard A.} and Forde, {Nancy R.}",
note = "Funding Information: Funding. The authors thank the following organizations for funding: Deutsche Forschungsgemeinschaft (DFG; postdoctoral fellowship to KL; Project ID: 415037474), the Fonds de recherche du Qu?bec ? Nature et technologies (FRQNT; postdoctoral fellowship to MS), and the Natural Sciences and Engineering Research Council of Canada (NSERC; Discovery Grant to NRF). Funding Information: The authors thank the following organizations for funding: Deutsche Forschungsgemeinschaft (DFG; postdoctoral fellowship to KL; Project ID: 415037474), the Fonds de recherche du Qu{\'e}bec – Nature et technologies (FRQNT; postdoctoral fellowship to MS), and the Natural Sciences and Engineering Research Council of Canada (NSERC; Discovery Grant to NRF). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2020 Lehmann, Shayegan, Blab and Forde. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = oct,
day = "6",
doi = "10.3389/fmolb.2020.577314",
language = "English",
volume = "7",
journal = "Frontiers in Molecular Biosciences",
issn = "2296-889X",
publisher = "Frontiers Media S.A.",
}