Optical readout of gold nanoparticle-based DNA microarrays without silver enhancement

Gerhard A. Blab; Laurent Cognet; Stéphane Berciaud; Isabelle Alexandre; Dieter Husar; José Remacle; Brahim Lounis

doi:10.1529/biophysj.105.076182

Optical readout of gold nanoparticle-based DNA microarrays without silver enhancement

Gerhard A. Blab, Laurent Cognet, Stéphane Berciaud, Isabelle Alexandre, Dieter Husar, José Remacle, Brahim Lounis^*

^*Corresponding author for this work

Sub Molecular Biophysics

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We present a novel readout scheme for gold nanoparticle-based DNA microarrays relying on "Laser-Induced Scattering around a NanoAbsorber". It provides direct counting of individual nanoparticles present on each array spot and stable signals, without any silver enhancement. Given the detection of nanometer-sized particles, which minimize the steric hindrance, the linear dynamic range of the method is particularly large and well suited for microarray detection.

Original language	English
Pages (from-to)	L13-L15
Journal	Biophysical Journal
Volume	90
Issue number	1
DOIs	https://doi.org/10.1529/biophysj.105.076182
Publication status	Published - Jan 2006

Bibliographical note

Funding Information:
G.A.B. acknowledges financial support by Fonds zur Förderung der wissenschaftlichen Forschung (FWF, Austria, Schrödinger-Stipendium) and the Fondation pour la Recherche Médicale (FRM, France). This research was funded by Centre National de la Recherche Scientifique (ACI Nanoscience and DRAB), Région Aquitaine, and the French Ministry for Education and Research (MENRT).

Funding

G.A.B. acknowledges financial support by Fonds zur Förderung der wissenschaftlichen Forschung (FWF, Austria, Schrödinger-Stipendium) and the Fondation pour la Recherche Médicale (FRM, France). This research was funded by Centre National de la Recherche Scientifique (ACI Nanoscience and DRAB), Région Aquitaine, and the French Ministry for Education and Research (MENRT).

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10.1529/biophysj.105.076182

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AU - Husar, Dieter

AU - Remacle, José

AU - Lounis, Brahim

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Optical readout of gold nanoparticle-based DNA microarrays without silver enhancement

Abstract

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