Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution

Massimiliano Berardi; Kevin Bielawski; Niek Rijnveld; Grzegorz Gruca; Hilde Aardema; Leni van Tol; Gijs Wuite; B Imran Akca

doi:10.1038/s42003-021-02121-1

Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution

Massimiliano Berardi
, Kevin Bielawski
, Niek Rijnveld
, Grzegorz Gruca
, Hilde Aardema
, Leni van Tol
, Gijs Wuite
, B Imran Akca

FAH Clinical Reproduction

University of Amsterdam, VU University Amsterdam
STI Outpatient Clinic, Public Health Service of Amsterdam (GGD Amsterdam), Amsterdam, The Netherlands.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develop a MPA system that measures pressure and displacement in real time with sub-nanometer resolution thanks to an interferometric readout. This highly sensitive MPA system enables studying the nanoscale behavior of soft biomaterials under tension and their frequency-dependent viscoelastic response.

Original language	English
Article number	610
Pages (from-to)	1-7
Number of pages	7
Journal	Communications Biology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/s42003-021-02121-1
Publication status	Published - Dec 2021

Bibliographical note

Funding Information:
This work was financially supported by the H2020 European Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement “Phys2BioMed” contract no. 812772. The authors would like to thank Stefan Werzinger and Noor Schilder for providing parts of the MATLAB code used to model the μGRIN lenses, Lily Kar-domateas for help in manufacturing pressure sensors, and Prof. Davide Iannuzzi for being the major driving force behind this work.

Publisher Copyright:
© 2021, The Author(s).

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s42003-021-02121-1Final published version, 2.09 MBLicence: CC BY

Cite this

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abstract = "Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develop a MPA system that measures pressure and displacement in real time with sub-nanometer resolution thanks to an interferometric readout. This highly sensitive MPA system enables studying the nanoscale behavior of soft biomaterials under tension and their frequency-dependent viscoelastic response.",

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doi = "10.1038/s42003-021-02121-1",

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AU - Berardi, Massimiliano

AU - Bielawski, Kevin

AU - Rijnveld, Niek

AU - Gruca, Grzegorz

AU - Aardema, Hilde

AU - van Tol, Leni

AU - Wuite, Gijs

AU - Akca, B Imran

N1 - Funding Information: This work was financially supported by the H2020 European Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement “Phys2BioMed” contract no. 812772. The authors would like to thank Stefan Werzinger and Noor Schilder for providing parts of the MATLAB code used to model the μGRIN lenses, Lily Kar-domateas for help in manufacturing pressure sensors, and Prof. Davide Iannuzzi for being the major driving force behind this work. Publisher Copyright: © 2021, The Author(s).

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N2 - Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develop a MPA system that measures pressure and displacement in real time with sub-nanometer resolution thanks to an interferometric readout. This highly sensitive MPA system enables studying the nanoscale behavior of soft biomaterials under tension and their frequency-dependent viscoelastic response.

AB - Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develop a MPA system that measures pressure and displacement in real time with sub-nanometer resolution thanks to an interferometric readout. This highly sensitive MPA system enables studying the nanoscale behavior of soft biomaterials under tension and their frequency-dependent viscoelastic response.

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Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution

Abstract

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