On-line coupling of cyclodextrin mediated nonaqueous capillary electrophoresis to mass spectrometry for the determination of salbutamol enantiomers in urine.

AC Servais, M Fillet, R. Mol, G.W. Somsen, P. Chiap, G.J. de Jong, J. Crommen

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The usefulness of the on-line coupling of nonaqueous capillary electrophoresis (NACE) with electrospray ionization (ESI) mass spectrometry (MS) using heptakis(2,3-di-O-acetyl-6-O-sulfo)-beta-cyclodextrin (HDAS-beta-CD) was demonstrated for the enantioselective determination of low concentrations of salbutamol in human urine. After optimization of several parameters, such as sheath-liquid composition and flow rate, nebulizing gas pressure, CE counter-pressure and position of the CE capillary outlet, a limit of quantification of 18 and 20 ng/ml was obtained for salbutamol enantiomers. Moreover, the relative standard deviation values for repeatability at a concentration of 30 ng/ml were below 7% for both enantiomers. Typical regression lines obtained after application of a simple linear regression model revealed a good relationship between peak area and analyte concentration (with 0.9988 and 0.9966 as coefficients of determination). This paper proposes an easy to use and sensitive NACE-MS method to determine enantiomers of a basic chiral drug in biological fluids preceded by solid-phase extraction as sample cleanup.
Original languageUndefined/Unknown
Pages (from-to)752-7
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Issue number3
Publication statusPublished - 2006


  • Medical technology
  • Farmacie(FARM)
  • Bescherming en bevordering van de menselijke gezondheid
  • Biomedische technologie en medicijnen
  • Public Health
  • Analytical chemistry
  • Drugmisbruik en verslaving

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