TY - JOUR
T1 - Occupational exposure to pesticides is associated with differential DNA methylation.
AU - van der Plaat, Diana A
AU - de Jong, Kim
AU - de Vries, Maaike
AU - van Diemen, Cleo C
AU - Nedeljkovic, Ivana
AU - Amin, Najaf
AU - Kromhout, Hans
AU - Vermeulen, Roel
AU - Postma, Dirkje S
AU - van Duijn, Cornelia M
AU - Boezen, H. Marike
AU - Vonk, Judith M
AU - Biobank-based Integrative Omics Study Consortium
PY - 2018/6
Y1 - 2018/6
N2 - OBJECTIVES: Occupational pesticide exposure is associated with a wide range of diseases, including lung diseases, but it is largely unknown how pesticides influence airway disease pathogenesis. A potential mechanism might be through epigenetic mechanisms, like DNA methylation. Therefore, we assessed associations between occupational exposure to pesticides and genome-wide DNA methylation sites. METHODS: 1561 subjects of LifeLines were included with either no (n=1392), low (n=108) or high (n=61) exposure to any type of pesticides (estimated based on current or last held job). Blood DNA methylation levels were measured using Illumina 450K arrays. Associations between pesticide exposure and 420 938 methylation sites (CpGs) were assessed using robust linear regression adjusted for appropriate confounders. In addition, we performed genome-wide stratified and interaction analyses by gender, smoking and airway obstruction status, and assessed associations between gene expression and methylation for genome-wide significant CpGs (n=2802). RESULTS: In total for all analyses, high pesticide exposure was genome-wide significantly (false discovery rate P
AB - OBJECTIVES: Occupational pesticide exposure is associated with a wide range of diseases, including lung diseases, but it is largely unknown how pesticides influence airway disease pathogenesis. A potential mechanism might be through epigenetic mechanisms, like DNA methylation. Therefore, we assessed associations between occupational exposure to pesticides and genome-wide DNA methylation sites. METHODS: 1561 subjects of LifeLines were included with either no (n=1392), low (n=108) or high (n=61) exposure to any type of pesticides (estimated based on current or last held job). Blood DNA methylation levels were measured using Illumina 450K arrays. Associations between pesticide exposure and 420 938 methylation sites (CpGs) were assessed using robust linear regression adjusted for appropriate confounders. In addition, we performed genome-wide stratified and interaction analyses by gender, smoking and airway obstruction status, and assessed associations between gene expression and methylation for genome-wide significant CpGs (n=2802). RESULTS: In total for all analyses, high pesticide exposure was genome-wide significantly (false discovery rate P
U2 - 10.1136/oemed-2017-104787
DO - 10.1136/oemed-2017-104787
M3 - Article
C2 - 29459480
SN - 1351-0711
VL - 75
SP - 427
EP - 435
JO - Occupational and Environmental Medicine
JF - Occupational and Environmental Medicine
IS - 6
ER -