Observation of inter-subunit nuclear Overhauser effects in a dimeric protein. Application to the Arc repressor

M.J.M. Burgering; R. Boelens; M. Caffrey; J.N. Breg; R. Kaptein

doi:10.1016/0014-5793(93)80929-O

Observation of inter-subunit nuclear Overhauser effects in a dimeric protein. Application to the Arc repressor

M.J.M. Burgering, R. Boelens, M. Caffrey, J.N. Breg, R. Kaptein

Bijvoet Ctr. for Biomolec. Research

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform 15N and 13C isotope labelling. This is accomplished by doubly filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc repressor and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.

Original language	English
Pages (from-to)	105-109
Number of pages	5
Journal	FEBS Letters
Volume	330
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(93)80929-O
Publication status	Published - 29 Jan 1993

Keywords

Arc repressor
dimer
inter-subunit NOE
NMR
article
nonhuman
nuclear magnetic resonance
priority journal
protein structure
repressor gene

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10.1016/0014-5793(93)80929-O

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abstract = "For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform 15N and 13C isotope labelling. This is accomplished by doubly filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc repressor and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.",

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AU - Burgering, M.J.M.

AU - Boelens, R.

AU - Caffrey, M.

AU - Breg, J.N.

AU - Kaptein, R.

PY - 1993/1/29

Y1 - 1993/1/29

N2 - For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform 15N and 13C isotope labelling. This is accomplished by doubly filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc repressor and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.

AB - For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform 15N and 13C isotope labelling. This is accomplished by doubly filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc repressor and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.

KW - Arc repressor

KW - dimer

KW - inter-subunit NOE

KW - NMR

KW - article

KW - nonhuman

KW - nuclear magnetic resonance

KW - priority journal

KW - protein structure

KW - repressor gene

U2 - 10.1016/0014-5793(93)80929-O

DO - 10.1016/0014-5793(93)80929-O

M3 - Article

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VL - 330

SP - 105

EP - 109

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Observation of inter-subunit nuclear Overhauser effects in a dimeric protein. Application to the Arc repressor

Abstract

Keywords

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