Observation of inter-subunit nuclear Overhauser effects in a dimeric protein. Application to the Arc repressor

M.J.M. Burgering, R. Boelens, M. Caffrey, J.N. Breg, R. Kaptein

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform 15N and 13C isotope labelling. This is accomplished by doubly filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc repressor and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.
Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalFEBS Letters
Volume330
Issue number1
DOIs
Publication statusPublished - 29 Jan 1993

Keywords

  • Arc repressor
  • dimer
  • inter-subunit NOE
  • NMR
  • article
  • nonhuman
  • nuclear magnetic resonance
  • priority journal
  • protein structure
  • repressor gene

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