Nuclear pore complex structure and dynamics revealed by cryoelectron tomography

Martin Beck; Friedrich Förster; Mary Ecke; Jürgen M Plitzko; Frauke Melchior; Günther Gerisch; Wolfgang Baumeister; Ohad Medalia

doi:10.1126/science.1104808

Nuclear pore complex structure and dynamics revealed by cryoelectron tomography

Martin Beck, Friedrich Förster, Mary Ecke, Jürgen M Plitzko, Frauke Melchior, Günther Gerisch, Wolfgang Baumeister, Ohad Medalia

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Abstract

Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes of the tomograms containing individual NPCs were extracted in silico and subjected to three-dimensional classification and averaging, whereby distinct structural states were observed. The central plug/transporter (CP/T) was variable in volume and could occupy different positions along the nucleocytoplasmic axis, which supports the notion that it essentially represents cargo in transit. Changes in the position of the CP/T were accompanied by structural rearrangements in the NPC scaffold.

Original language	English
Pages (from-to)	1387-90
Number of pages	4
Journal	Science
Volume	306
Issue number	5700
DOIs	https://doi.org/10.1126/science.1104808
Publication status	Published - 19 Nov 2004
Externally published	Yes

Keywords

Active Transport, Cell Nucleus
Animals
Cell Nucleus
Cryoelectron Microscopy
Cytoskeleton
Dictyostelium
Image Processing, Computer-Assisted
Imaging, Three-Dimensional
Nuclear Pore
Tomography

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title = "Nuclear pore complex structure and dynamics revealed by cryoelectron tomography",

abstract = "Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes of the tomograms containing individual NPCs were extracted in silico and subjected to three-dimensional classification and averaging, whereby distinct structural states were observed. The central plug/transporter (CP/T) was variable in volume and could occupy different positions along the nucleocytoplasmic axis, which supports the notion that it essentially represents cargo in transit. Changes in the position of the CP/T were accompanied by structural rearrangements in the NPC scaffold.",

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TY - JOUR

T1 - Nuclear pore complex structure and dynamics revealed by cryoelectron tomography

AU - Beck, Martin

AU - Förster, Friedrich

AU - Ecke, Mary

AU - Plitzko, Jürgen M

AU - Melchior, Frauke

AU - Gerisch, Günther

AU - Baumeister, Wolfgang

AU - Medalia, Ohad

PY - 2004/11/19

Y1 - 2004/11/19

N2 - Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes of the tomograms containing individual NPCs were extracted in silico and subjected to three-dimensional classification and averaging, whereby distinct structural states were observed. The central plug/transporter (CP/T) was variable in volume and could occupy different positions along the nucleocytoplasmic axis, which supports the notion that it essentially represents cargo in transit. Changes in the position of the CP/T were accompanied by structural rearrangements in the NPC scaffold.

AB - Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes of the tomograms containing individual NPCs were extracted in silico and subjected to three-dimensional classification and averaging, whereby distinct structural states were observed. The central plug/transporter (CP/T) was variable in volume and could occupy different positions along the nucleocytoplasmic axis, which supports the notion that it essentially represents cargo in transit. Changes in the position of the CP/T were accompanied by structural rearrangements in the NPC scaffold.

KW - Active Transport, Cell Nucleus

KW - Animals

KW - Cell Nucleus

KW - Cryoelectron Microscopy

KW - Cytoskeleton

KW - Dictyostelium

KW - Image Processing, Computer-Assisted

KW - Imaging, Three-Dimensional

KW - Nuclear Pore

KW - Tomography

U2 - 10.1126/science.1104808

DO - 10.1126/science.1104808

M3 - Article

C2 - 15514115

SN - 0036-8075

VL - 306

SP - 1387

EP - 1390

JO - Science

JF - Science

IS - 5700

ER -

Nuclear pore complex structure and dynamics revealed by cryoelectron tomography

Abstract

Keywords

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