TY - JOUR
T1 - Novel Type II Collagen Reporter Mice
T2 - New Tool for Assessing Collagen 2 alpha 1 Expression In Vivo and In Vitro
AU - Tryfonidou, Marianna A.
AU - Lunstrum, Gregory P.
AU - Hendriks, Kristyanne
AU - Riemers, Frank M.
AU - Wubbolts, Richard
AU - Hazewinkel, H. A. W.
AU - Degnin, Catherine R.
AU - Horton, William A.
PY - 2011/3
Y1 - 2011/3
N2 - We report the generation of a new mouse strain harboring a Col2-pd2EGFP reporter transgene; pd2EGFP has a much shorter half-life than EGFP, making it a near real-time reporter for Col2 alpha 1 expression in vivo and in vitro. In the post-natal growth plate, pd2EGFP fluorescence was expressed in almost all proliferative chondrocytes and in some hypertrophic chondrocytes based on localization with type X collagen. In articular cartilage, pd2EGFP fluorescence diminished over time, nicely illustrating the decrease of type II collagen synthesis in articular chondrocytes during growth. Monolayers of FACS-sorted chondrocytes from P1-2 mice showed faster loss of pd2EGFP compared to EGFP, reflecting rapid chondrocyte de-differentiation. High-density culture of FACS-pd2EGFP- growth plate chondrocytes revealed the typical temporal expression pattern in which type II collagen preceded type X collagen matrix deposition. The Col2-pd2EGFP reporter mouse will be a valuable tool for studies of growth plate chondrocyte biology. Developmental Dynamics 240:663-673, 2011. (C) 2011 Wiley-Liss, Inc.
AB - We report the generation of a new mouse strain harboring a Col2-pd2EGFP reporter transgene; pd2EGFP has a much shorter half-life than EGFP, making it a near real-time reporter for Col2 alpha 1 expression in vivo and in vitro. In the post-natal growth plate, pd2EGFP fluorescence was expressed in almost all proliferative chondrocytes and in some hypertrophic chondrocytes based on localization with type X collagen. In articular cartilage, pd2EGFP fluorescence diminished over time, nicely illustrating the decrease of type II collagen synthesis in articular chondrocytes during growth. Monolayers of FACS-sorted chondrocytes from P1-2 mice showed faster loss of pd2EGFP compared to EGFP, reflecting rapid chondrocyte de-differentiation. High-density culture of FACS-pd2EGFP- growth plate chondrocytes revealed the typical temporal expression pattern in which type II collagen preceded type X collagen matrix deposition. The Col2-pd2EGFP reporter mouse will be a valuable tool for studies of growth plate chondrocyte biology. Developmental Dynamics 240:663-673, 2011. (C) 2011 Wiley-Liss, Inc.
KW - Col2 alpha 1
KW - Gfp
KW - Chondrocyte
KW - Growth plate
KW - Reporter mice
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=d7dz6a2i7wiom976oc9ff2iqvdhv8k5x&SrcAuth=WosAPI&KeyUT=WOS:000288033500016&DestLinkType=FullRecord&DestApp=WOS
U2 - 10.1002/dvdy.22569
DO - 10.1002/dvdy.22569
M3 - Article
C2 - 21337464
SN - 1058-8388
VL - 240
SP - 663
EP - 673
JO - Developmental Dynamics
JF - Developmental Dynamics
IS - 3
ER -