Novel O-linked carbohydrate chains in the cellulase complex (cellulose) of Clostridium thermocellum. 3-O-methyl-N-acetylglucosamine as a constituent of a glycoprotein

G.J. Gerwig; P. De Waard; J.P. Kamerling; J.F.G. Vliegenthart; E. Morgenstern; R. Lamed; E.A. Bayer

Novel O-linked carbohydrate chains in the cellulase complex (cellulose) of Clostridium thermocellum. 3-O-methyl-N-acetylglucosamine as a constituent of a glycoprotein

G.J. Gerwig
, P. De Waard
, J.P. Kamerling
, J.F.G. Vliegenthart
, E. Morgenstern
, R. Lamed
, E.A. Bayer

Bijvoet Center

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Alkaline borohydride treatment of the cellulosome of Clostridium thermocellum yielded two major oligosaccharide-alditols. The compounds, isolated via gel permeation chromatography and high performance liquid chromatography, were analyzed by monosaccharide analysis, methylation analysis, gas-liquid chromatography/mass spectrometry, fast atom bombardment/mass spectrometry, and one- and two-dimensional 500-MHz (COSY, HOHAHA, ROESY) 1H NMR spectroscopy. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with blotting technology indicated that the tetrasaccharide is mainly associated with one of the cellulosome subunits.

Original language	English
Pages (from-to)	1027-1035
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	264
Issue number	2
Publication status	Published - 15 Jul 1989

Keywords

cellulase
carbohydrate analysis
Clostridium thermocellum
nonhuman
priority journal

Cite this

@article{72c0ecd694ec40f8b4fcac8bca6f63f8,

title = "Novel O-linked carbohydrate chains in the cellulase complex (cellulose) of Clostridium thermocellum. 3-O-methyl-N-acetylglucosamine as a constituent of a glycoprotein",

abstract = "Alkaline borohydride treatment of the cellulosome of Clostridium thermocellum yielded two major oligosaccharide-alditols. The compounds, isolated via gel permeation chromatography and high performance liquid chromatography, were analyzed by monosaccharide analysis, methylation analysis, gas-liquid chromatography/mass spectrometry, fast atom bombardment/mass spectrometry, and one- and two-dimensional 500-MHz (COSY, HOHAHA, ROESY) 1H NMR spectroscopy. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with blotting technology indicated that the tetrasaccharide is mainly associated with one of the cellulosome subunits.",

keywords = "cellulase, carbohydrate analysis, Clostridium thermocellum, nonhuman, priority journal",

author = "G.J. Gerwig and \{De Waard\}, P. and J.P. Kamerling and J.F.G. Vliegenthart and E. Morgenstern and R. Lamed and E.A. Bayer",

year = "1989",

month = jul,

day = "15",

language = "English",

volume = "264",

pages = "1027--1035",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "2",

}

Novel O-linked carbohydrate chains in the cellulase complex (cellulose) of Clostridium thermocellum. 3-O-methyl-N-acetylglucosamine as a constituent of a glycoprotein. / Gerwig, G.J.; De Waard, P.; Kamerling, J.P. et al.
In: Journal of Biological Chemistry, Vol. 264, No. 2, 15.07.1989, p. 1027-1035.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Novel O-linked carbohydrate chains in the cellulase complex (cellulose) of Clostridium thermocellum. 3-O-methyl-N-acetylglucosamine as a constituent of a glycoprotein

AU - Gerwig, G.J.

AU - De Waard, P.

AU - Kamerling, J.P.

AU - Vliegenthart, J.F.G.

AU - Morgenstern, E.

AU - Lamed, R.

AU - Bayer, E.A.

PY - 1989/7/15

Y1 - 1989/7/15

N2 - Alkaline borohydride treatment of the cellulosome of Clostridium thermocellum yielded two major oligosaccharide-alditols. The compounds, isolated via gel permeation chromatography and high performance liquid chromatography, were analyzed by monosaccharide analysis, methylation analysis, gas-liquid chromatography/mass spectrometry, fast atom bombardment/mass spectrometry, and one- and two-dimensional 500-MHz (COSY, HOHAHA, ROESY) 1H NMR spectroscopy. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with blotting technology indicated that the tetrasaccharide is mainly associated with one of the cellulosome subunits.

AB - Alkaline borohydride treatment of the cellulosome of Clostridium thermocellum yielded two major oligosaccharide-alditols. The compounds, isolated via gel permeation chromatography and high performance liquid chromatography, were analyzed by monosaccharide analysis, methylation analysis, gas-liquid chromatography/mass spectrometry, fast atom bombardment/mass spectrometry, and one- and two-dimensional 500-MHz (COSY, HOHAHA, ROESY) 1H NMR spectroscopy. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis combined with blotting technology indicated that the tetrasaccharide is mainly associated with one of the cellulosome subunits.

KW - cellulase

KW - carbohydrate analysis

KW - Clostridium thermocellum

KW - nonhuman

KW - priority journal

M3 - Article

SN - 0021-9258

VL - 264

SP - 1027

EP - 1035

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 2

ER -

Novel O-linked carbohydrate chains in the cellulase complex (cellulose) of Clostridium thermocellum. 3-O-methyl-N-acetylglucosamine as a constituent of a glycoprotein

Abstract

Keywords

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