Novel carbapenemases FLC-1 and IMI-2 encoded by an enterobacter cloacae complex isolated from food products

Michael S.M. Brouwer; Kamaleddin H.M.E. Tehrani; Michel Rapallini; Yvon Geurts; Arie Kant; Frank Harders; Vida Mashayekhi; Nathaniel I. Martin; Alex Bossers; Dik J. Mevius; Ben Wit; Kees T. Veldman

doi:10.1128/AAC.02338-18

Novel carbapenemases FLC-1 and IMI-2 encoded by an enterobacter cloacae complex isolated from food products

Michael S.M. Brouwer^*, Kamaleddin H.M.E. Tehrani, Michel Rapallini, Yvon Geurts, Arie Kant, Frank Harders, Vida Mashayekhi, Nathaniel I. Martin, Alex Bossers, Dik J. Mevius, Ben Wit, Kees T. Veldman

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Food for human consumption is screened widely for the presence of antibiotic-resistant bacteria to assess the potential for transfer of resistant bacteria to the general population. Here, we describe an Enterobacter cloacae complex isolated from imported seafood that encodes two carbapenemases on two distinct plasmids. Both enzymes belong to Ambler class A -lactamases, the previously described IMI-2 and a novel family designated FLC-1. The hydrolytic activity of the novel enzyme against aminopenicillins, cephalosporins, and carbapenems was determined.

Original language	English
Article number	e02338-18
Journal	Antimicrobial Agents and Chemotherapy
Volume	63
Issue number	6
DOIs	https://doi.org/10.1128/AAC.02338-18
Publication status	Published - 1 Jun 2019

Funding

The work was funded by the Ministry of Agriculture, Nature and Food Quality (BO-43-013.03-002) and by the Netherlands Food and Consumer Product Safety Authority. M.S.M.B., K.H.M.E.T., N.I.M., B.W., and K.T.V. conceived and designed the experiments.

Keywords

Antimicrobial resistance
Carbapenemase
Enterobacter
Plasmid

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AAC.02338-18

Antimicrobial Agents and Chemotherapy-2019-Brouwer-e02338-18.fullFinal published version, 1.16 MB

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Brouwer, M. S. M., Tehrani, K. H. M. E., Rapallini, M., Geurts, Y., Kant, A., Harders, F., Mashayekhi, V., Martin, N. I., Bossers, A., Mevius, D. J., Wit, B., & Veldman, K. T. (2019). Novel carbapenemases FLC-1 and IMI-2 encoded by an enterobacter cloacae complex isolated from food products. Antimicrobial Agents and Chemotherapy, 63(6), Article e02338-18. https://doi.org/10.1128/AAC.02338-18

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abstract = "Food for human consumption is screened widely for the presence of antibiotic-resistant bacteria to assess the potential for transfer of resistant bacteria to the general population. Here, we describe an Enterobacter cloacae complex isolated from imported seafood that encodes two carbapenemases on two distinct plasmids. Both enzymes belong to Ambler class A -lactamases, the previously described IMI-2 and a novel family designated FLC-1. The hydrolytic activity of the novel enzyme against aminopenicillins, cephalosporins, and carbapenems was determined.",

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AU - Brouwer, Michael S.M.

AU - Tehrani, Kamaleddin H.M.E.

AU - Rapallini, Michel

AU - Geurts, Yvon

AU - Kant, Arie

AU - Harders, Frank

AU - Mashayekhi, Vida

AU - Martin, Nathaniel I.

AU - Bossers, Alex

AU - Mevius, Dik J.

AU - Wit, Ben

AU - Veldman, Kees T.

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AB - Food for human consumption is screened widely for the presence of antibiotic-resistant bacteria to assess the potential for transfer of resistant bacteria to the general population. Here, we describe an Enterobacter cloacae complex isolated from imported seafood that encodes two carbapenemases on two distinct plasmids. Both enzymes belong to Ambler class A -lactamases, the previously described IMI-2 and a novel family designated FLC-1. The hydrolytic activity of the novel enzyme against aminopenicillins, cephalosporins, and carbapenems was determined.

KW - Antimicrobial resistance

KW - Carbapenemase

KW - Enterobacter

KW - Plasmid

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Novel carbapenemases FLC-1 and IMI-2 encoded by an enterobacter cloacae complex isolated from food products

Abstract

Funding

Keywords

UN SDGs

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