NMR cross-correlated relaxation rates reveal ion coordination sites in DNA

R. Fiala, N. Spackova, S. Foldynová-Trantírková, J. Sponer, V. Sklenár, L. Trantirek

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In this work, a novel NMR method for the identification of preferential coordination sites between physiologically relevant counterions and nucleic acid bases is demonstrated. In this approach, the NMR cross-correlated relaxation rates between the aromatic carbon chemical shift anisotropy and the proton–carbon dipolar interaction are monitored as a function of increasing Na+, K+, and Mg2+ concentrations. Increasing the counterion concentration modulates the residence times of the counterions at specific sites around the nucleic acid bases. It is demonstrated that the modulation of the counterion concentration leads to sizable variations of the cross-correlated relaxation rates, which can be used to probe the site-specific counterion coordination. In parallel, the very same measurements report on the rotational tumbling of DNA, which, as shown here, depends on the nature of the ion and its concentration. This methodology is highly sensitive and easily implemented. The method can be used to cross-validate and/or complement direct but artifact-prone experimental techniques such as X-ray diffraction, NMR analysis with substitutionary ions, and molecular dynamics simulations. The feasibility of this technique is demonstrated on the extraordinarily stable DNA mini-hairpin d(GCGAAGC).
Original languageEnglish
Pages (from-to)13790-13793
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number35
DOIs
Publication statusPublished - 2011

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