NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field

Eline J. Koers; Elwin A. W. van der Cruijsen; Melanie Rosay; Markus Weingarth; Alexander Prokofyev; Claire Sauvee; Olivier Ouari; Johan van der Zwan; Olaf Pongs; Paul Tordo; Werner E. Maas; Marc Baldus

doi:10.1007/s10858-014-9865-8

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field

Eline J. Koers
, Elwin A. W. van der Cruijsen
, Melanie Rosay
, Markus Weingarth
, Alexander Prokofyev
, Claire Sauvee
, Olivier Ouari
, Johan van der Zwan
, Olaf Pongs
, Paul Tordo
, Werner E. Maas
, Marc Baldus^*

^*Corresponding author for this work

Bruker BioSpin Corp
CNRS Centre National de la Recherche Scientifique
Saarland University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400 MHz/263 GHz), high field DNP (800 MHz/527 GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural probes under magic angle spinning conditions. Applied to a membrane-embedded K+ channel, this approach allowed us to refine the membrane-embedded channel structure and revealed conformational substates that are present during two different stages of the channel gating cycle. High-field DNP thus offers atomic insight into the role of molecular plasticity during the course of biomolecular function in a complex cellular environment.

Original language	English
Pages (from-to)	157-168
Number of pages	12
Journal	Journal of Biomolecular NMR
Volume	60
Issue number	2-3
DOIs	https://doi.org/10.1007/s10858-014-9865-8
Publication status	Published - Nov 2014

Funding

We thank Mark Daniels for excellent technical support. This work was supported by NWO (grants 722.012.002 to MW and 700.11.344 and 700.58.102 to MB), DFG (Po137, 40-1 and 41-1) and NIH (NIH/NIGNS grant GM087519).

Keywords

NMR
Dynamic nuclear polarization
Membrane
Protein
Solid-state NMR
SOLID-STATE NMR
C-TYPE INACTIVATION
K+-CHANNEL
POTASSIUM CHANNEL
RESONANCE-SPECTROSCOPY
SELECTIVITY FILTER
SIGNAL ENHANCEMENT
LIPID-BILAYERS
HIGH-FREQUENCY
RELAXATION

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title = "NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field",

abstract = "Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400 MHz/263 GHz), high field DNP (800 MHz/527 GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural probes under magic angle spinning conditions. Applied to a membrane-embedded K+ channel, this approach allowed us to refine the membrane-embedded channel structure and revealed conformational substates that are present during two different stages of the channel gating cycle. High-field DNP thus offers atomic insight into the role of molecular plasticity during the course of biomolecular function in a complex cellular environment.",

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author = "Koers, \{Eline J.\} and \{van der Cruijsen\}, \{Elwin A. W.\} and Melanie Rosay and Markus Weingarth and Alexander Prokofyev and Claire Sauvee and Olivier Ouari and \{van der Zwan\}, Johan and Olaf Pongs and Paul Tordo and Maas, \{Werner E.\} and Marc Baldus",

year = "2014",

month = nov,

doi = "10.1007/s10858-014-9865-8",

language = "English",

volume = "60",

pages = "157--168",

journal = "Journal of Biomolecular NMR",

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number = "2-3",

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AU - Koers, Eline J.

AU - van der Cruijsen, Elwin A. W.

AU - Rosay, Melanie

AU - Weingarth, Markus

AU - Prokofyev, Alexander

AU - Sauvee, Claire

AU - Ouari, Olivier

AU - van der Zwan, Johan

AU - Pongs, Olaf

AU - Tordo, Paul

AU - Maas, Werner E.

AU - Baldus, Marc

PY - 2014/11

Y1 - 2014/11

N2 - Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400 MHz/263 GHz), high field DNP (800 MHz/527 GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural probes under magic angle spinning conditions. Applied to a membrane-embedded K+ channel, this approach allowed us to refine the membrane-embedded channel structure and revealed conformational substates that are present during two different stages of the channel gating cycle. High-field DNP thus offers atomic insight into the role of molecular plasticity during the course of biomolecular function in a complex cellular environment.

AB - Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400 MHz/263 GHz), high field DNP (800 MHz/527 GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural probes under magic angle spinning conditions. Applied to a membrane-embedded K+ channel, this approach allowed us to refine the membrane-embedded channel structure and revealed conformational substates that are present during two different stages of the channel gating cycle. High-field DNP thus offers atomic insight into the role of molecular plasticity during the course of biomolecular function in a complex cellular environment.

KW - NMR

KW - Dynamic nuclear polarization

KW - Membrane

KW - Protein

KW - Solid-state NMR

KW - SOLID-STATE NMR

KW - C-TYPE INACTIVATION

KW - K+-CHANNEL

KW - POTASSIUM CHANNEL

KW - RESONANCE-SPECTROSCOPY

KW - SELECTIVITY FILTER

KW - SIGNAL ENHANCEMENT

KW - LIPID-BILAYERS

KW - HIGH-FREQUENCY

KW - RELAXATION

U2 - 10.1007/s10858-014-9865-8

DO - 10.1007/s10858-014-9865-8

M3 - Article

SN - 0925-2738

VL - 60

SP - 157

EP - 168

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

IS - 2-3

ER -

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field

Abstract

Funding

Keywords

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