TY - JOUR
T1 - Next-generation proteomics: towards an integrative view of proteome dynamics
AU - Altelaar, A.F.M.
AU - Munoz-Peralta, J.
AU - Heck, A.J.R.
PY - 2013
Y1 - 2013
N2 - Next-generation sequencing allows the analysis of genomes, including those representing disease states. However, the causes of most disorders are multifactorial, and systems-level approaches, including the analysis of proteomes, are required for a more comprehensive understanding. The proteome is extremely multifaceted owing to splicing and protein modifications, and this is further amplified by the interconnectivity of proteins into complexes and signalling networks that are highly divergent in time and space. Proteome analysis heavily relies on mass spectrometry (MS). MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis. Here we describe this emerging next generation of proteomics and highlight recent applications.
AB - Next-generation sequencing allows the analysis of genomes, including those representing disease states. However, the causes of most disorders are multifactorial, and systems-level approaches, including the analysis of proteomes, are required for a more comprehensive understanding. The proteome is extremely multifaceted owing to splicing and protein modifications, and this is further amplified by the interconnectivity of proteins into complexes and signalling networks that are highly divergent in time and space. Proteome analysis heavily relies on mass spectrometry (MS). MS-based proteomics is starting to mature and to deliver through a combination of developments in instrumentation, sample preparation and computational analysis. Here we describe this emerging next generation of proteomics and highlight recent applications.
U2 - 10.1038/nrg3356
DO - 10.1038/nrg3356
M3 - Article
SN - 1471-0056
VL - 14
SP - 35
EP - 48
JO - Nature Reviews. Genetics
JF - Nature Reviews. Genetics
ER -