New properties of wheat bran: anti-biofilm activity and interference with bacteria quorum-sensing systems

Gemma González-Ortiz, H C Quarles Van Ufford, S Bart A Halkes, Marta Cerdà-Cuéllar, Cees J Beukelman, Roland J Pieters, Rob M J Liskamp, José F Pérez, Susana M Martín-Orue

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Some plant extracts, have been demonstrated to interfere with the microbial metabolism of several pathogenic bacteria. Within this antimicrobial properties it has been described the potential to inhibit or destroy biofilms or to interfere in quorum-sensing (QS) systems. However, to our knowledge, no study exploring this potential of wheat-bran (WB) has been published. The purpose of the present study is to evaluate the anti-biofilm activity of WB against a cow mastitis strain of Staphylococcus aureus and also its possible interference with bacterial QS systems. The potential of inhibition and destruction of the biofilm was studied by different in vitro assays. Also, we tested the ability of WB to interfere in bacterial QS by degrading acyl-homoserine lactones (AHL) as one of the most studied QS signal molecules for Gram-negative bacteria. The soluble extract of WB at 0.5% showed anti-biofilm activity, inhibiting biofilm formation and also destroying it. Similarly, the > 300 kDa fraction from WB had significant anti-biofilm activity in both in vitro assays. The WB also showed a potential to interfere with bacterial QS systems, as it was demonstrated to contain certain lactonase activity able to reduce AHL concentration in the medium. The present study reveals two additional beneficial properties of WB extract never explored before, which may be related to the presence of defence compounds in the plant extract able to interfere with microbial biofilms and also QS systems.

    Original languageEnglish
    Pages (from-to)1346-1353
    Number of pages8
    JournalEnvironmental Microbiology
    Volume16
    Issue number5
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Acyl-Butyrolactones
    • Anti-Bacterial Agents
    • Biofilms
    • Dietary Fiber
    • Gram-Negative Bacteria
    • Plant Extracts
    • Quorum Sensing
    • Staphylococcus aureus

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