Abstract
In the current study we show the dissociation and tumor accumulation dynamics of dual-labeled near-infrared quantum dot core self-assembled lipidic nanoparticles (SALNPs) in a mouse model upon intravenous administration. Using advanced in vivo fluorescence energy transfer imaging techniques, we observed swift exchange with plasma protein components in the blood and progressive SALNP dissociation and subsequent trafficking of individual SALNP components following tumor accumulation. Our results suggest that upon intravenous administration SALNPs quickly transform, which may affect their functionality. The presented technology provides a modular in vivo tool to visualize SALNP behavior in real time and may contribute to improving the therapeutic outcome or molecular imaging signature of SALNPs.
Original language | English |
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Pages (from-to) | 10362-10370 |
Number of pages | 9 |
Journal | ACS Nano |
Volume | 7 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2013 |
Funding
This work was financially supported by the division of Chemical Sciences (CW) of the Dutch Science Foundation (NWO) under grant number ECHO.06.B.047 (A.M.) and the National Heart, Lung, and Blood Institute, National Institutes of Health, as a Program of Excellence in Nanotechnology (PEN) Award, Contract #HHSN268201000045C, the NIH grants R01EB009638 (Z.A.F.), R01CA155432 (W.J.M.M.), and R21CA159075 (A.R.), and the Norwegian Cancer Society. We thank Yu Zhou for his help with fluorescence imaging and K. Saeterbo for implanting the dorsal window chambers in mice and culturing cells.
Keywords
- quantum dots
- Forster resonance energy transfer
- in vivo imaging
- tumor-bearing mice
- lipidic nanoparticles
- QUANTUM DOTS
- IN-VIVO
- CONTRAST AGENTS
- NANOMEDICINE
- NANOCRYSTALS
- DELIVERY
- PLATFORM
- SURFACE