Near-Infrared Fluorescence Energy Transfer Imaging of Nanoparticle Accumulation and Dissociation Kinetics in Tumor-Bearing Mice

Yiming Zhao, Inge van Rooy, Sjoerd Hak, Francois Fay, Jun Tang, Catharina de Lange Davies, Mihaela Skobe, Edward Allen Fisher, Aurelian Radu, Zahi A. Fayad, Celso de Mello Donega, Andries Meijerink, Willem J. M. Mulder*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In the current study we show the dissociation and tumor accumulation dynamics of dual-labeled near-infrared quantum dot core self-assembled lipidic nanoparticles (SALNPs) in a mouse model upon intravenous administration. Using advanced in vivo fluorescence energy transfer imaging techniques, we observed swift exchange with plasma protein components in the blood and progressive SALNP dissociation and subsequent trafficking of individual SALNP components following tumor accumulation. Our results suggest that upon intravenous administration SALNPs quickly transform, which may affect their functionality. The presented technology provides a modular in vivo tool to visualize SALNP behavior in real time and may contribute to improving the therapeutic outcome or molecular imaging signature of SALNPs.

Original languageEnglish
Pages (from-to)10362-10370
Number of pages9
JournalACS Nano
Volume7
Issue number11
DOIs
Publication statusPublished - Nov 2013

Funding

This work was financially supported by the division of Chemical Sciences (CW) of the Dutch Science Foundation (NWO) under grant number ECHO.06.B.047 (A.M.) and the National Heart, Lung, and Blood Institute, National Institutes of Health, as a Program of Excellence in Nanotechnology (PEN) Award, Contract #HHSN268201000045C, the NIH grants R01EB009638 (Z.A.F.), R01CA155432 (W.J.M.M.), and R21CA159075 (A.R.), and the Norwegian Cancer Society. We thank Yu Zhou for his help with fluorescence imaging and K. Saeterbo for implanting the dorsal window chambers in mice and culturing cells.

Keywords

  • quantum dots
  • Forster resonance energy transfer
  • in vivo imaging
  • tumor-bearing mice
  • lipidic nanoparticles
  • QUANTUM DOTS
  • IN-VIVO
  • CONTRAST AGENTS
  • NANOMEDICINE
  • NANOCRYSTALS
  • DELIVERY
  • PLATFORM
  • SURFACE

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