Nanoscale β-TCP-Laden GelMA/PCL Composite Membrane for Guided Bone Regeneration

Abdel H Mahmoud; Yuanyuan Han; Renan Dal-Fabbro; Arwa Daghrery; Jinping Xu; Darnell Kaigler; Sarit B Bhaduri; Jos Malda; Marco C Bottino

doi:10.1021/acsami.3c03059

Nanoscale β-TCP-Laden GelMA/PCL Composite Membrane for Guided Bone Regeneration

Abdel H Mahmoud, Yuanyuan Han, Renan Dal-Fabbro, Arwa Daghrery, Jinping Xu, Darnell Kaigler, Sarit B Bhaduri, Jos Malda, Marco C Bottino^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Major advances in the field of periodontal tissue engineering have favored the fabrication of biodegradable membranes with tunable physical and biological properties for guided bone regeneration (GBR). Herein, we engineered innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes via electrospinning. Chemo-morphological findings showed that the composite microfibers had a uniform porous network and β-TCP particles successfully integrated within the fibers. Compared with pure PCL and GelMA/PCL, GelMA/PCL-TCP membranes led to increased cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs). Moreover, our GelMA/PCL-TCP membrane was able to promote robust bone regeneration in rat calvarial critical-size defects, showing remarkable osteogenesis compared to PCL and GelMA/PCL groups. Altogether, the GelMA/PCL-TCP composite fibrous membrane promoted osteogenic differentiation of aBMSCs in vitro and pronounced bone formation in vivo. Our data confirmed that the electrospun GelMA/PCL-TCP composite has a strong potential as a promising membrane for guided bone regeneration.

Original language	English
Pages (from-to)	32121–32135
Number of pages	15
Journal	ACS applied materials & interfaces
Volume	15
Issue number	27
Early online date	26 Jun 2023
DOIs	https://doi.org/10.1021/acsami.3c03059
Publication status	Published - 12 Jul 2023

Bibliographical note

Funding Information:
M.C.B. acknowledges the National Institutes of Health (NIH – National Institute of Dental and Craniofacial Research, grant R01DE031476). D.K. acknowledges NIH/NIDCR, grant R01DE028657). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH and the National Science Foundation (NSF). J.M. acknowledges the funding from the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research (024.003.013).

Publisher Copyright:
© 2023 American Chemical Society.

Keywords

bone
electrospinning
extracellular matrix
gelatin
regeneration
tissue engineering

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10.1021/acsami.3c03059

acsami.3c03059Final published version, 14.5 MBLicence: Taverne

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title = "Nanoscale β-TCP-Laden GelMA/PCL Composite Membrane for Guided Bone Regeneration",

abstract = "Major advances in the field of periodontal tissue engineering have favored the fabrication of biodegradable membranes with tunable physical and biological properties for guided bone regeneration (GBR). Herein, we engineered innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes via electrospinning. Chemo-morphological findings showed that the composite microfibers had a uniform porous network and β-TCP particles successfully integrated within the fibers. Compared with pure PCL and GelMA/PCL, GelMA/PCL-TCP membranes led to increased cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs). Moreover, our GelMA/PCL-TCP membrane was able to promote robust bone regeneration in rat calvarial critical-size defects, showing remarkable osteogenesis compared to PCL and GelMA/PCL groups. Altogether, the GelMA/PCL-TCP composite fibrous membrane promoted osteogenic differentiation of aBMSCs in vitro and pronounced bone formation in vivo. Our data confirmed that the electrospun GelMA/PCL-TCP composite has a strong potential as a promising membrane for guided bone regeneration.",

keywords = "bone, electrospinning, extracellular matrix, gelatin, regeneration, tissue engineering",

author = "Mahmoud, \{Abdel H\} and Yuanyuan Han and Renan Dal-Fabbro and Arwa Daghrery and Jinping Xu and Darnell Kaigler and Bhaduri, \{Sarit B\} and Jos Malda and Bottino, \{Marco C\}",

note = "Funding Information: M.C.B. acknowledges the National Institutes of Health (NIH – National Institute of Dental and Craniofacial Research, grant R01DE031476). D.K. acknowledges NIH/NIDCR, grant R01DE028657). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH and the National Science Foundation (NSF). J.M. acknowledges the funding from the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research (024.003.013). Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",

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doi = "10.1021/acsami.3c03059",

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AU - Mahmoud, Abdel H

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AU - Dal-Fabbro, Renan

AU - Daghrery, Arwa

AU - Xu, Jinping

AU - Kaigler, Darnell

AU - Bhaduri, Sarit B

AU - Malda, Jos

AU - Bottino, Marco C

N1 - Funding Information: M.C.B. acknowledges the National Institutes of Health (NIH – National Institute of Dental and Craniofacial Research, grant R01DE031476). D.K. acknowledges NIH/NIDCR, grant R01DE028657). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH and the National Science Foundation (NSF). J.M. acknowledges the funding from the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research (024.003.013). Publisher Copyright: © 2023 American Chemical Society.

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Y1 - 2023/7/12

N2 - Major advances in the field of periodontal tissue engineering have favored the fabrication of biodegradable membranes with tunable physical and biological properties for guided bone regeneration (GBR). Herein, we engineered innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes via electrospinning. Chemo-morphological findings showed that the composite microfibers had a uniform porous network and β-TCP particles successfully integrated within the fibers. Compared with pure PCL and GelMA/PCL, GelMA/PCL-TCP membranes led to increased cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs). Moreover, our GelMA/PCL-TCP membrane was able to promote robust bone regeneration in rat calvarial critical-size defects, showing remarkable osteogenesis compared to PCL and GelMA/PCL groups. Altogether, the GelMA/PCL-TCP composite fibrous membrane promoted osteogenic differentiation of aBMSCs in vitro and pronounced bone formation in vivo. Our data confirmed that the electrospun GelMA/PCL-TCP composite has a strong potential as a promising membrane for guided bone regeneration.

AB - Major advances in the field of periodontal tissue engineering have favored the fabrication of biodegradable membranes with tunable physical and biological properties for guided bone regeneration (GBR). Herein, we engineered innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes via electrospinning. Chemo-morphological findings showed that the composite microfibers had a uniform porous network and β-TCP particles successfully integrated within the fibers. Compared with pure PCL and GelMA/PCL, GelMA/PCL-TCP membranes led to increased cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs). Moreover, our GelMA/PCL-TCP membrane was able to promote robust bone regeneration in rat calvarial critical-size defects, showing remarkable osteogenesis compared to PCL and GelMA/PCL groups. Altogether, the GelMA/PCL-TCP composite fibrous membrane promoted osteogenic differentiation of aBMSCs in vitro and pronounced bone formation in vivo. Our data confirmed that the electrospun GelMA/PCL-TCP composite has a strong potential as a promising membrane for guided bone regeneration.

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KW - extracellular matrix

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JO - ACS applied materials & interfaces

JF - ACS applied materials & interfaces

IS - 27

ER -

Nanoscale β-TCP-Laden GelMA/PCL Composite Membrane for Guided Bone Regeneration

Abstract

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Keywords

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