N-glycoproteomics in plants: perspectives and challenges

W. Song; M.G.L. Henquet; R.A. Mentink; Aalt Jan van Dijk; J.H.G. Cordewener; H.J. Bosch; A.H.P. America; A.R. van der Krol

doi:10.1016/j.jprot.2011.05.007

N-glycoproteomics in plants: perspectives and challenges

W. Song, M.G.L. Henquet, R.A. Mentink, Aalt Jan van Dijk, J.H.G. Cordewener, H.J. Bosch, A.H.P. America, A.R. van der Krol

Sub Membrane Biochemistry & Biophysics

extern

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In eukaryotes, proteins that are secreted into the ER are mostly modified by N-glycans on consensus NxS/T sites. The N-linked glycan subsequently undergoes varying degrees of processing by enzymes which are spatially distributed over the ER and the Golgi apparatus. The post-ER N-glycan processing to complex glycans differs between animals and plants, with consequences for N-glycan and glycopeptide isolation and characterization of plant glycoproteins. Here we describe some recent developments in plant glycoproteomics and illustrate how general and plant specific technologies may be used to address different important biological questions.

Original language	English
Pages (from-to)	1463-1474
Number of pages	10
Journal	Journal of Proteomics
Volume	74
Issue number	8
DOIs	https://doi.org/10.1016/j.jprot.2011.05.007
Publication status	Published - 2011

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AU - Song, W.

AU - Henquet, M.G.L.

AU - Mentink, R.A.

AU - van Dijk, Aalt Jan

AU - Cordewener, J.H.G.

AU - Bosch, H.J.

AU - America, A.H.P.

AU - van der Krol, A.R.

PY - 2011

Y1 - 2011

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AB - In eukaryotes, proteins that are secreted into the ER are mostly modified by N-glycans on consensus NxS/T sites. The N-linked glycan subsequently undergoes varying degrees of processing by enzymes which are spatially distributed over the ER and the Golgi apparatus. The post-ER N-glycan processing to complex glycans differs between animals and plants, with consequences for N-glycan and glycopeptide isolation and characterization of plant glycoproteins. Here we describe some recent developments in plant glycoproteomics and illustrate how general and plant specific technologies may be used to address different important biological questions.

U2 - 10.1016/j.jprot.2011.05.007

DO - 10.1016/j.jprot.2011.05.007

M3 - Article

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VL - 74

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EP - 1474

JO - Journal of Proteomics

JF - Journal of Proteomics

IS - 8

ER -

N-glycoproteomics in plants: perspectives and challenges

Abstract

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