Abstract
Objective. Although extracellular matrix (ECM)–derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro. Design. Scaffolds were generated from decellularized equine articular cartilage and seeded with either chondrocytes or multipotent stromal cells (MSCs). After 2, 4, and 6 weeks of in vitro culture, CDM constructs were analyzed both histologically (hematoxylin and eosin, Safranin-O, collagen types I and II) and biochemically (glycosaminoglycan [GAG] and DNA content). Results. After 4 weeks, both cell types demonstrated chondrogenic differentiation; however, the MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix. Conclusion. These promising in vitro results underscore the potency of CDM scaffolds in (osteo)chondral defect repair.
Original language | English |
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Pages (from-to) | 221-230 |
Number of pages | 10 |
Journal | Cartilage |
Volume | 5 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- cartilage tissue engineering
- chondrocyte
- extracellular matrix (ECM)
- mesenchymal stromal cell
- scaffold