Multiphoton intravital microscopy of rodents

Colinda L.G.J. Scheele; David Herrmann; Erika Yamashita; Cristina Lo Celso; Craig N. Jenne; Maja H. Oktay; David Entenberg; Peter Friedl; Roberto Weigert; Franck L.B. Meijboom; Masaru Ishii; Paul Timpson; Jacco van Rheenen

doi:10.1038/s43586-022-00168-w

Multiphoton intravital microscopy of rodents

Colinda L.G.J. Scheele, David Herrmann, Erika Yamashita, Cristina Lo Celso, Craig N. Jenne, Maja H. Oktay, David Entenberg, Peter Friedl, Roberto Weigert, Franck L.B. Meijboom, Masaru Ishii, Paul Timpson^*, Jacco van Rheenen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.

Original language	English
Article number	89
Number of pages	26
Journal	Nature Reviews Methods Primers
Volume	2
Issue number	1
DOIs	https://doi.org/10.1038/s43586-022-00168-w
Publication status	Published - 10 Nov 2022

Bibliographical note

Funding Information:
C.L.G.J.S. was supported by a European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 1035-2020), a Federation of the European Biochemical Societies (FEBS) excellence award and an Excellence of Science (EOS) grant (project ID: 40007532) of Fonds Wetenschappelijk Onderzoek — Le Fonds de la Recherche Scientifique (FWO-FNRS). P.T. was supported by the Len Ainsworth Fellowship in Pancreatic Cancer Research and is a National Health and Medical Research Council (NHMRC) Senior Research Fellow. D.H. was supported by a Cancer Institute NSW (CINSW) Early Career Research Fellowship. R.W. was supported by the National Institutes of Health (NIH), National Cancer Institute (NCI) Center for Cancer Research Intramural Research Program (ZIA BC 011682). C.N.J. was supported by the Canada Research Chairs Program. P.F. was supported by the NIH U54 CA261694-01 and ERC-2021-ADG 101054921. J.v.R. was supported by the VICI (09150182110004) of ZonMW of the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) and the Doctor Josef Steiner Foundation. J.v.R. and P.F. were also funded by the CancerGenomics.nl (Netherlands Organization for Scientific Research) programme. D.E. and M.H.O. are supported by the NCI (CA255153) and The Gruss-Lipper Biophotonics Center and its associated Integrated Imaging Program, and by Jane A. and Myles P. Dempsey.

Publisher Copyright:
© 2022, Springer Nature Limited.

Funding

C.L.G.J.S. was supported by a European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 1035-2020), a Federation of the European Biochemical Societies (FEBS) excellence award and an Excellence of Science (EOS) grant (project ID: 40007532) of Fonds Wetenschappelijk Onderzoek — Le Fonds de la Recherche Scientifique (FWO-FNRS). P.T. was supported by the Len Ainsworth Fellowship in Pancreatic Cancer Research and is a National Health and Medical Research Council (NHMRC) Senior Research Fellow. D.H. was supported by a Cancer Institute NSW (CINSW) Early Career Research Fellowship. R.W. was supported by the National Institutes of Health (NIH), National Cancer Institute (NCI) Center for Cancer Research Intramural Research Program (ZIA BC 011682). C.N.J. was supported by the Canada Research Chairs Program. P.F. was supported by the NIH U54 CA261694-01 and ERC-2021-ADG 101054921. J.v.R. was supported by the VICI (09150182110004) of ZonMW of the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) and the Doctor Josef Steiner Foundation. J.v.R. and P.F. were also funded by the CancerGenomics.nl (Netherlands Organization for Scientific Research) programme. D.E. and M.H.O. are supported by the NCI (CA255153) and The Gruss-Lipper Biophotonics Center and its associated Integrated Imaging Program, and by Jane A. and Myles P. Dempsey.

Keywords

Hematopoietic stem-cells
Neutrophil extracellular traps
Disseminated tumor-cells
Time imaging reveals
Dynamics in-vivo
Bone-marrow
Real-time
Fluorescence recovery
2-photon excitation
Lymph-nodes

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10.1038/s43586-022-00168-w

s43586-022-00168-wFinal published version, 5.98 MBLicence: Taverne

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abstract = "Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.",

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note = "Funding Information: C.L.G.J.S. was supported by a European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 1035-2020), a Federation of the European Biochemical Societies (FEBS) excellence award and an Excellence of Science (EOS) grant (project ID: 40007532) of Fonds Wetenschappelijk Onderzoek — Le Fonds de la Recherche Scientifique (FWO-FNRS). P.T. was supported by the Len Ainsworth Fellowship in Pancreatic Cancer Research and is a National Health and Medical Research Council (NHMRC) Senior Research Fellow. D.H. was supported by a Cancer Institute NSW (CINSW) Early Career Research Fellowship. R.W. was supported by the National Institutes of Health (NIH), National Cancer Institute (NCI) Center for Cancer Research Intramural Research Program (ZIA BC 011682). C.N.J. was supported by the Canada Research Chairs Program. P.F. was supported by the NIH U54 CA261694-01 and ERC-2021-ADG 101054921. J.v.R. was supported by the VICI (09150182110004) of ZonMW of the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) and the Doctor Josef Steiner Foundation. J.v.R. and P.F. were also funded by the CancerGenomics.nl (Netherlands Organization for Scientific Research) programme. D.E. and M.H.O. are supported by the NCI (CA255153) and The Gruss-Lipper Biophotonics Center and its associated Integrated Imaging Program, and by Jane A. and Myles P. Dempsey. Publisher Copyright: {\textcopyright} 2022, Springer Nature Limited.",

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Multiphoton intravital microscopy of rodents

Abstract

Bibliographical note

Funding

Keywords

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