TY - JOUR
T1 - Multiphoton intravital microscopy of rodents
AU - Scheele, Colinda L.G.J.
AU - Herrmann, David
AU - Yamashita, Erika
AU - Lo Celso, Cristina
AU - Jenne, Craig N.
AU - Oktay, Maja H.
AU - Entenberg, David
AU - Friedl, Peter
AU - Weigert, Roberto
AU - Meijboom, Franck L.B.
AU - Ishii, Masaru
AU - Timpson, Paul
AU - van Rheenen, Jacco
N1 - Funding Information:
C.L.G.J.S. was supported by a European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 1035-2020), a Federation of the European Biochemical Societies (FEBS) excellence award and an Excellence of Science (EOS) grant (project ID: 40007532) of Fonds Wetenschappelijk Onderzoek — Le Fonds de la Recherche Scientifique (FWO-FNRS). P.T. was supported by the Len Ainsworth Fellowship in Pancreatic Cancer Research and is a National Health and Medical Research Council (NHMRC) Senior Research Fellow. D.H. was supported by a Cancer Institute NSW (CINSW) Early Career Research Fellowship. R.W. was supported by the National Institutes of Health (NIH), National Cancer Institute (NCI) Center for Cancer Research Intramural Research Program (ZIA BC 011682). C.N.J. was supported by the Canada Research Chairs Program. P.F. was supported by the NIH U54 CA261694-01 and ERC-2021-ADG 101054921. J.v.R. was supported by the VICI (09150182110004) of ZonMW of the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO) and the Doctor Josef Steiner Foundation. J.v.R. and P.F. were also funded by the CancerGenomics.nl (Netherlands Organization for Scientific Research) programme. D.E. and M.H.O. are supported by the NCI (CA255153) and The Gruss-Lipper Biophotonics Center and its associated Integrated Imaging Program, and by Jane A. and Myles P. Dempsey.
Publisher Copyright:
© 2022, Springer Nature Limited.
PY - 2022/11/10
Y1 - 2022/11/10
N2 - Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.
AB - Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.
KW - Hematopoietic stem-cells
KW - Neutrophil extracellular traps
KW - Disseminated tumor-cells
KW - Time imaging reveals
KW - Dynamics in-vivo
KW - Bone-marrow
KW - Real-time
KW - Fluorescence recovery
KW - 2-photon excitation
KW - Lymph-nodes
UR - http://www.scopus.com/inward/record.url?scp=85141847297&partnerID=8YFLogxK
U2 - 10.1038/s43586-022-00168-w
DO - 10.1038/s43586-022-00168-w
M3 - Article
AN - SCOPUS:85141847297
SN - 2662-8449
VL - 2
JO - Nature Reviews Methods Primers
JF - Nature Reviews Methods Primers
IS - 1
M1 - 89
ER -