Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae

S M Bruisten; G T Noordhoek; A J C van den Brule; B Duim; C H E Boel; K El-Faouzi; R du Maine; S Mulder; D Luijt; J Schirm

doi:10.1128/JCM.42.9.4332-4334.2004

Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae

S M Bruisten, G T Noordhoek, A J C van den Brule, B Duim, C H E Boel, K El-Faouzi, R du Maine, S Mulder, D Luijt, J Schirm

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.

Original language	English
Pages (from-to)	4332-4
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	42
Issue number	9
DOIs	https://doi.org/10.1128/JCM.42.9.4332-4334.2004
Publication status	Published - Sept 2004

Keywords

Bacterial Outer Membrane Proteins
Humans
Neisseria gonorrhoeae
Polymerase Chain Reaction
Reproducibility of Results

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10.1128/JCM.42.9.4332-4334.2004

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title = "Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae",

abstract = "The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.",

keywords = "Bacterial Outer Membrane Proteins, Humans, Neisseria gonorrhoeae, Polymerase Chain Reaction, Reproducibility of Results",

author = "Bruisten, {S M} and Noordhoek, {G T} and {van den Brule}, {A J C} and B Duim and Boel, {C H E} and K El-Faouzi and {du Maine}, R and S Mulder and D Luijt and J Schirm",

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doi = "10.1128/JCM.42.9.4332-4334.2004",

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TY - JOUR

T1 - Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae

AU - Bruisten, S M

AU - Noordhoek, G T

AU - van den Brule, A J C

AU - Duim, B

AU - Boel, C H E

AU - El-Faouzi, K

AU - du Maine, R

AU - Mulder, S

AU - Luijt, D

AU - Schirm, J

PY - 2004/9

Y1 - 2004/9

N2 - The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.

AB - The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.

KW - Bacterial Outer Membrane Proteins

KW - Humans

KW - Neisseria gonorrhoeae

KW - Polymerase Chain Reaction

KW - Reproducibility of Results

U2 - 10.1128/JCM.42.9.4332-4334.2004

DO - 10.1128/JCM.42.9.4332-4334.2004

M3 - Article

C2 - 15365038

SN - 0095-1137

VL - 42

SP - 4332

EP - 4334

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 9

ER -

Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae

Abstract

Keywords

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