Abstract
An unprecedented generic system allowing the 3D printing of peptide-functionalized hydrogels by soft sol-gel inorganic polymerization is presented. Hybrid silylated inorganic/bioorganic blocks are mixed in biological buffer in an appropriate ratio, to yield a multicomponent bioink that can be printed as a hydrogel without using any photochemical or organic reagent. Hydrolysis and condensation of the silylated precursors occur during the printing process and result in a covalent network in which molecules are linked through siloxane bonds. The viscosity of the colloidal solution used as bioink was monitored in order to set up the optimal conditions for extrusion printing. Grid-patterned hydrogel scaffolds containing a hybrid integrin ligand were printed using a pressure-driven rapid prototyping machine. Finally, they were seeded with mesenchymal stem cells, demonstrating their suitability for cell culture. The versatility of the sol-gel process and its biocompatibility makes this approach highly promising for the preparation of tailor-made cell-laden scaffolds.
| Original language | English |
|---|---|
| Pages (from-to) | 12231-12235 |
| Number of pages | 5 |
| Journal | RSC Advances |
| Volume | 7 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 21 Feb 2017 |
| Externally published | Yes |
Bibliographical note
Funding Information:C. Echalier's PhD was partly founded by the “Région Languedoc Roussillon” through the program “Chercheur d'Avenir”, grant attributed to G. Subra. Peptide synthesis and purification were performed using SynBio3 platform facilities supported by GIS IBISA and ITMO Cancer. The 3D scaffold fabrication was performed using the facilities of the platform of Production of Biomaterials and Biomolecules of the ICTS “NANBIOSIS”, more specifically by the U5 Unit of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at the Institute for Bioenginering of Catalonia (IBEC).
Publisher Copyright:
© The Royal Society of Chemistry.
Funding
C. Echalier's PhD was partly founded by the “Région Languedoc Roussillon” through the program “Chercheur d'Avenir”, grant attributed to G. Subra. Peptide synthesis and purification were performed using SynBio3 platform facilities supported by GIS IBISA and ITMO Cancer. The 3D scaffold fabrication was performed using the facilities of the platform of Production of Biomaterials and Biomolecules of the ICTS “NANBIOSIS”, more specifically by the U5 Unit of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at the Institute for Bioenginering of Catalonia (IBEC).