Mobility and solvent exposure of aromatic residues in bacteriorhodopsin investigated by 1H-NMR and photo-CIDNP-NMR spectroscopy

K.H. Mayo; A. Schussheim; G.W. Vuister; R. Boelens; R. Kaptein; M. Engelhard; B. Hess

doi:10.1016/0014-5793(88)81255-9

Mobility and solvent exposure of aromatic residues in bacteriorhodopsin investigated by 1H-NMR and photo-CIDNP-NMR spectroscopy

K.H. Mayo, A. Schussheim, G.W. Vuister, R. Boelens, R. Kaptein, M. Engelhard, B. Hess

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Proton-NMR studies of native bacteriorhodopsin revealed the existence of about nine relatively mobile aromatic residues out of the 32 totally present in the protein. These nine comprise approximately three tyrosines, two tryptophans and four phenylalanines. Photo-CIDNP data strongly suggest that, aside from phenylalanines, only one tyrosine and one tryptophan residue are exposed to the solvent. These data are discussed in terms of the current structural model for bacteriorhodopsin.

Original language	English
Pages (from-to)	163-168
Number of pages	6
Journal	FEBS Letters
Volume	235
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(88)81255-9
Publication status	Published - 1 Aug 1988

Keywords

Bacteriorhodopsin
Membrane protein
NMR
Photo-CIDNP
Proton nuclear resonance
Side-chain mobility
exposure
model
nuclear magnetic resonance spectroscopy
proton nuclear magnetic resonance
bacteriorhodopsin
solvent

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10.1016/0014-5793(88)81255-9

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title = "Mobility and solvent exposure of aromatic residues in bacteriorhodopsin investigated by 1H-NMR and photo-CIDNP-NMR spectroscopy",

abstract = "Proton-NMR studies of native bacteriorhodopsin revealed the existence of about nine relatively mobile aromatic residues out of the 32 totally present in the protein. These nine comprise approximately three tyrosines, two tryptophans and four phenylalanines. Photo-CIDNP data strongly suggest that, aside from phenylalanines, only one tyrosine and one tryptophan residue are exposed to the solvent. These data are discussed in terms of the current structural model for bacteriorhodopsin.",

keywords = "Bacteriorhodopsin, Membrane protein, NMR, Photo-CIDNP, Proton nuclear resonance, Side-chain mobility, exposure, model, nuclear magnetic resonance spectroscopy, proton nuclear magnetic resonance, bacteriorhodopsin, solvent",

author = "K.H. Mayo and A. Schussheim and G.W. Vuister and R. Boelens and R. Kaptein and M. Engelhard and B. Hess",

year = "1988",

month = aug,

day = "1",

doi = "10.1016/0014-5793(88)81255-9",

language = "English",

volume = "235",

pages = "163--168",

journal = "FEBS Letters",

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publisher = "John Wiley and Sons Inc.",

number = "1-2",

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TY - JOUR

T1 - Mobility and solvent exposure of aromatic residues in bacteriorhodopsin investigated by 1H-NMR and photo-CIDNP-NMR spectroscopy

AU - Mayo, K.H.

AU - Schussheim, A.

AU - Vuister, G.W.

AU - Boelens, R.

AU - Kaptein, R.

AU - Engelhard, M.

AU - Hess, B.

PY - 1988/8/1

Y1 - 1988/8/1

N2 - Proton-NMR studies of native bacteriorhodopsin revealed the existence of about nine relatively mobile aromatic residues out of the 32 totally present in the protein. These nine comprise approximately three tyrosines, two tryptophans and four phenylalanines. Photo-CIDNP data strongly suggest that, aside from phenylalanines, only one tyrosine and one tryptophan residue are exposed to the solvent. These data are discussed in terms of the current structural model for bacteriorhodopsin.

AB - Proton-NMR studies of native bacteriorhodopsin revealed the existence of about nine relatively mobile aromatic residues out of the 32 totally present in the protein. These nine comprise approximately three tyrosines, two tryptophans and four phenylalanines. Photo-CIDNP data strongly suggest that, aside from phenylalanines, only one tyrosine and one tryptophan residue are exposed to the solvent. These data are discussed in terms of the current structural model for bacteriorhodopsin.

KW - Bacteriorhodopsin

KW - Membrane protein

KW - NMR

KW - Photo-CIDNP

KW - Proton nuclear resonance

KW - Side-chain mobility

KW - exposure

KW - model

KW - nuclear magnetic resonance spectroscopy

KW - proton nuclear magnetic resonance

KW - bacteriorhodopsin

KW - solvent

U2 - 10.1016/0014-5793(88)81255-9

DO - 10.1016/0014-5793(88)81255-9

M3 - Article

SN - 0014-5793

VL - 235

SP - 163

EP - 168

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Mobility and solvent exposure of aromatic residues in bacteriorhodopsin investigated by 1H-NMR and photo-CIDNP-NMR spectroscopy

Abstract

Keywords

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