Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles

Jan Lötvall; Andrew F Hill; Fred Hochberg; Edit I Buzás; Dolores Di Vizio; Christopher Gardiner; Yong Song Gho; Igor V Kurochkin; Suresh Mathivanan; Peter Quesenberry; Susmita Sahoo; Hidetoshi Tahara; Marca H Wauben; Kenneth W Witwer; Clotilde Théry

doi:10.3402/jev.v3.26913

Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles

Jan Lötvall, Andrew F Hill, Fred Hochberg, Edit I Buzás, Dolores Di Vizio, Christopher Gardiner, Yong Song Gho, Igor V Kurochkin, Suresh Mathivanan, Peter Quesenberry, Susmita Sahoo, Hidetoshi Tahara, Marca H Wauben, Kenneth W Witwer, Clotilde Théry

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.

Original language	English
Pages (from-to)	26913
Journal	Journal of Extracellular Vesicles
Volume	3
DOIs	https://doi.org/10.3402/jev.v3.26913
Publication status	Published - 2014

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Lötvall, J., Hill, A. F., Hochberg, F., Buzás, E. I., Di Vizio, D., Gardiner, C., Gho, Y. S., Kurochkin, I. V., Mathivanan, S., Quesenberry, P., Sahoo, S., Tahara, H., Wauben, M. H., Witwer, K. W., & Théry, C. (2014). Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. Journal of Extracellular Vesicles, 3, 26913. https://doi.org/10.3402/jev.v3.26913

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abstract = "Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.",

author = "Jan L{\"o}tvall and Hill, {Andrew F} and Fred Hochberg and Buz{\'a}s, {Edit I} and {Di Vizio}, Dolores and Christopher Gardiner and Gho, {Yong Song} and Kurochkin, {Igor V} and Suresh Mathivanan and Peter Quesenberry and Susmita Sahoo and Hidetoshi Tahara and Wauben, {Marca H} and Witwer, {Kenneth W} and Clotilde Th{\'e}ry",

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doi = "10.3402/jev.v3.26913",

language = "English",

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Lötvall, J, Hill, AF, Hochberg, F, Buzás, EI, Di Vizio, D, Gardiner, C, Gho, YS, Kurochkin, IV, Mathivanan, S, Quesenberry, P, Sahoo, S, Tahara, H, Wauben, MH, Witwer, KW & Théry, C 2014, 'Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles', Journal of Extracellular Vesicles, vol. 3, pp. 26913. https://doi.org/10.3402/jev.v3.26913

Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. / Lötvall, Jan; Hill, Andrew F; Hochberg, Fred et al.
In: Journal of Extracellular Vesicles, Vol. 3, 2014, p. 26913.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Minimal experimental requirements for definition of extracellular vesicles and their functions

T2 - a position statement from the International Society for Extracellular Vesicles

AU - Lötvall, Jan

AU - Hill, Andrew F

AU - Hochberg, Fred

AU - Buzás, Edit I

AU - Di Vizio, Dolores

AU - Gardiner, Christopher

AU - Gho, Yong Song

AU - Kurochkin, Igor V

AU - Mathivanan, Suresh

AU - Quesenberry, Peter

AU - Sahoo, Susmita

AU - Tahara, Hidetoshi

AU - Wauben, Marca H

AU - Witwer, Kenneth W

AU - Théry, Clotilde

PY - 2014

Y1 - 2014

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AB - Secreted membrane-enclosed vesicles, collectively called extracellular vesicles (EVs), which include exosomes, ectosomes, microvesicles, microparticles, apoptotic bodies and other EV subsets, encompass a very rapidly growing scientific field in biology and medicine. Importantly, it is currently technically challenging to obtain a totally pure EV fraction free from non-vesicular components for functional studies, and therefore there is a need to establish guidelines for analyses of these vesicles and reporting of scientific studies on EV biology. Here, the International Society for Extracellular Vesicles (ISEV) provides researchers with a minimal set of biochemical, biophysical and functional standards that should be used to attribute any specific biological cargo or functions to EVs.

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DO - 10.3402/jev.v3.26913

M3 - Article

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SN - 2001-3078

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JO - Journal of Extracellular Vesicles

JF - Journal of Extracellular Vesicles

ER -

Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles

Abstract

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