Mimicking the bovine oviduct in a microfluidic device for advanced embryo in vitro culture systems

Marcia Ferraz, Hoon Suk Rho, Juliette Delahaye, Nuno Pinheiro, Heiko Henning, Tom Stout, Bart Gadella, Séverine Le Gac

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Here, we report a microfluidic device acting as an oviduct for in vitro fertilization of bovine oocytes followed by the embryo pre-implantation culture. The platform consists of two fluidic compartments separated by a porous membrane onto which oviduct epithelial cells are culture, to recapitulate the microniche found in the oviduct to which gametes and pre-implantation embryos are exposed. We demonstrate superior performance of this platform in terms of epigenetic patterns of in vitro produced embryos compared to conventional culture dishes.
Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages1047-1048
Number of pages2
ISBN (Print)9780692941836
Publication statusPublished - 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Keywords

  • Embryo culture
  • Epigenetics
  • Microfluidics
  • Organ-on-a-chip platforms
  • Oviduct

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