Microtubule stabilization reduces scarring and causes axon regeneration after spinal cord injury

Farida Hellal; Andres Hurtado; Jörg Ruschel; Kevin C Flynn; Claudia J Laskowski; Martina Umlauf; Lukas C Kapitein; Dinara Strikis; Vance Lemmon; John Bixby; Casper C Hoogenraad; Frank Bradke

doi:10.1126/science.1201148

Microtubule stabilization reduces scarring and causes axon regeneration after spinal cord injury

Farida Hellal, Andres Hurtado, Jörg Ruschel, Kevin C Flynn, Claudia J Laskowski, Martina Umlauf, Lukas C Kapitein, Dinara Strikis, Vance Lemmon, John Bixby, Casper C Hoogenraad, Frank Bradke

Sub Cell Biology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Hypertrophic scarring and poor intrinsic axon growth capacity constitute major obstacles for spinal cord repair. These processes are tightly regulated by microtubule dynamics. Here, moderate microtubule stabilization decreased scar formation after spinal cord injury in rodents through various cellular mechanisms, including dampening of transforming growth factor-β signaling. It prevented accumulation of chondroitin sulfate proteoglycans and rendered the lesion site permissive for axon regeneration of growth-competent sensory neurons. Microtubule stabilization also promoted growth of central nervous system axons of the Raphe-spinal tract and led to functional improvement. Thus, microtubule stabilization reduces fibrotic scarring and enhances the capacity of axons to grow.

Original language	English
Pages (from-to)	928-931
Number of pages	4
Journal	Science
Volume	331
Issue number	6019
DOIs	https://doi.org/10.1126/science.1201148
Publication status	Published - 18 Feb 2011

Keywords

Animals
Axons
Cells, Cultured
Chondroitin Sulfate Proteoglycans
Cicatrix
Female
Ganglia, Spinal
Kinesin
Microtubules
Paclitaxel
Protein Transport
Rats
Rats, Sprague-Dawley
Sensory Receptor Cells
Signal Transduction
Smad2 Protein
Spinal Cord
Spinal Cord Injuries
Spinal Cord Regeneration
Transforming Growth Factor beta

Access to Document

10.1126/science.1201148

Cite this

@article{6d34e14e5d3b4a60b4c96e419f68bba8,

title = "Microtubule stabilization reduces scarring and causes axon regeneration after spinal cord injury",

abstract = "Hypertrophic scarring and poor intrinsic axon growth capacity constitute major obstacles for spinal cord repair. These processes are tightly regulated by microtubule dynamics. Here, moderate microtubule stabilization decreased scar formation after spinal cord injury in rodents through various cellular mechanisms, including dampening of transforming growth factor-β signaling. It prevented accumulation of chondroitin sulfate proteoglycans and rendered the lesion site permissive for axon regeneration of growth-competent sensory neurons. Microtubule stabilization also promoted growth of central nervous system axons of the Raphe-spinal tract and led to functional improvement. Thus, microtubule stabilization reduces fibrotic scarring and enhances the capacity of axons to grow.",

keywords = "Animals, Axons, Cells, Cultured, Chondroitin Sulfate Proteoglycans, Cicatrix, Female, Ganglia, Spinal, Kinesin, Microtubules, Paclitaxel, Protein Transport, Rats, Rats, Sprague-Dawley, Sensory Receptor Cells, Signal Transduction, Smad2 Protein, Spinal Cord, Spinal Cord Injuries, Spinal Cord Regeneration, Transforming Growth Factor beta",

author = "Farida Hellal and Andres Hurtado and J{\"o}rg Ruschel and Flynn, {Kevin C} and Laskowski, {Claudia J} and Martina Umlauf and Kapitein, {Lukas C} and Dinara Strikis and Vance Lemmon and John Bixby and Hoogenraad, {Casper C} and Frank Bradke",

year = "2011",

month = feb,

day = "18",

doi = "10.1126/science.1201148",

language = "English",

volume = "331",

pages = "928--931",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6019",

}

TY - JOUR

T1 - Microtubule stabilization reduces scarring and causes axon regeneration after spinal cord injury

AU - Hellal, Farida

AU - Hurtado, Andres

AU - Ruschel, Jörg

AU - Flynn, Kevin C

AU - Laskowski, Claudia J

AU - Umlauf, Martina

AU - Kapitein, Lukas C

AU - Strikis, Dinara

AU - Lemmon, Vance

AU - Bixby, John

AU - Hoogenraad, Casper C

AU - Bradke, Frank

PY - 2011/2/18

Y1 - 2011/2/18

N2 - Hypertrophic scarring and poor intrinsic axon growth capacity constitute major obstacles for spinal cord repair. These processes are tightly regulated by microtubule dynamics. Here, moderate microtubule stabilization decreased scar formation after spinal cord injury in rodents through various cellular mechanisms, including dampening of transforming growth factor-β signaling. It prevented accumulation of chondroitin sulfate proteoglycans and rendered the lesion site permissive for axon regeneration of growth-competent sensory neurons. Microtubule stabilization also promoted growth of central nervous system axons of the Raphe-spinal tract and led to functional improvement. Thus, microtubule stabilization reduces fibrotic scarring and enhances the capacity of axons to grow.

AB - Hypertrophic scarring and poor intrinsic axon growth capacity constitute major obstacles for spinal cord repair. These processes are tightly regulated by microtubule dynamics. Here, moderate microtubule stabilization decreased scar formation after spinal cord injury in rodents through various cellular mechanisms, including dampening of transforming growth factor-β signaling. It prevented accumulation of chondroitin sulfate proteoglycans and rendered the lesion site permissive for axon regeneration of growth-competent sensory neurons. Microtubule stabilization also promoted growth of central nervous system axons of the Raphe-spinal tract and led to functional improvement. Thus, microtubule stabilization reduces fibrotic scarring and enhances the capacity of axons to grow.

KW - Animals

KW - Axons

KW - Cells, Cultured

KW - Chondroitin Sulfate Proteoglycans

KW - Cicatrix

KW - Female

KW - Ganglia, Spinal

KW - Kinesin

KW - Microtubules

KW - Paclitaxel

KW - Protein Transport

KW - Rats

KW - Rats, Sprague-Dawley

KW - Sensory Receptor Cells

KW - Signal Transduction

KW - Smad2 Protein

KW - Spinal Cord

KW - Spinal Cord Injuries

KW - Spinal Cord Regeneration

KW - Transforming Growth Factor beta

U2 - 10.1126/science.1201148

DO - 10.1126/science.1201148

M3 - Article

C2 - 21273450

SN - 0036-8075

VL - 331

SP - 928

EP - 931

JO - Science

JF - Science

IS - 6019

ER -

Microtubule stabilization reduces scarring and causes axon regeneration after spinal cord injury

Abstract

Keywords

Access to Document

Fingerprint

Cite this