Microbial products from probiotic bacteria inhibit Salmonella enteritidis 857-induced IL-8 synthesis in Caco-2 cells

J J Malago, E Nemeth, J F J G Koninkx, P C J Tooten, S Fajdiga, J E van Dijk

    Research output: Contribution to journalArticleAcademicpeer-review


    Oral administration of Lactobacillus spp. as probiotics is gaining importance in the treatment of intestinal inflammations. However, their mechanism of action is unknown. We investigated whether nonspecific binding Lactobacillus casei Shirota (LcS) and mannose-specific Lactobacillus plantarum 299v (Lp) and their spent culture supernatant (SCS) affect Salmonella enteritidis 857 (Se) growth, IL-8 and Hsp70 syntheses. In one set of experiments human enterocyte-like Caco-2 cells were infected with LcS, Lp or Se at 1-500 bacteria per cell for 1 h. In another set, cells were exposed to Se (0-200 per cell, 1 h) after exposure to lactobacilli (LB) (500 per cell, 30 min) or by co-incubation of Se and LB (1 h). The third set of experiments involved exposure of cells for 1 h to SCS or Se (100 per cell) pretreated (1 h) in SCS. The effect of LB SCS on Se growth was evaluated by agar plate diffusion test. IL-8 and Hsp70 were assessed over 2-24 h using ELISA and Western blotting, respectively. Neither LcS nor Lp affected the Se growth and IL-8 production. In addition, they did not induce Hsp70 expression by Caco-2 cells. Instead, their SCS inhibited the Se growth and IL-8 production and induced the expression of Hsp70 by both crypt- and villus-like cells. The beneficial effect of Lactobacillus spp. to the intestinal inflammations might be associated with a decrease in IL-8 levels. This effect could be mediated, at least in part, via a secreted antimicrobial product(s) either directly against the pathogens or indirectly through the synthesis of Hsp70.

    Original languageEnglish
    Pages (from-to)401-408
    Number of pages8
    JournalFolia Microbiologica
    Issue number4
    Publication statusPublished - Jul 2010


    • Blotting, Western
    • Caco-2 Cells
    • Coculture Techniques
    • Culture Media
    • Enzyme-Linked Immunosorbent Assay
    • HSP70 Heat-Shock Proteins
    • Humans
    • Immunologic Factors
    • Interleukin-8
    • Lactobacillus casei
    • Lactobacillus plantarum
    • Probiotics
    • Salmonella enteritidis


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